人类外周血淋巴细胞对A组链球菌疫苗的增殖反应。

The proliferative response of human peripheral blood lymphocytes to group A streptococcal vaccine.

作者信息

Morell A, Stoffel-Mazenauer U, Vassalli G, Riesen W F, Braun D G

出版信息

Clin Immunol Immunopathol. 1987 Jun;43(3):314-24. doi: 10.1016/0090-1229(87)90141-3.

Abstract

The proliferative response of human peripheral blood mononuclear cells to group A streptococcal vaccine (strain J17A4) was assessed by measurement of [3H]thymidine incorporation. This was maximal after 7 days in cultures containing 1 X 10(5) lymphocytes. Cells from 33 out of 38 adult donors (87%) yielded stimulation indices between 2.0 and 81. Responding cells were predominantly of the OKT4+, Ia+, Tac+ phenotype expressed by activated helper T lymphocytes. In contrast, no response was observed in 10 out of 12 samples of cord blood lymphocytes. Addition of conventional rabbit or monoclonal murine anti-Ia (HLA-DR) antibodies to the culture medium abrogated the vaccine-induced proliferative response. Frequencies of responding cells estimated by limiting dilution analysis were between 1 in 2370 and 1 in 4580 lymphocytes. The results suggest that lymphocyte stimulations induced with streptococcal group A vaccine represented an in vitro anamnestic cellular immune response.

摘要

通过测量[3H]胸腺嘧啶核苷掺入量，评估人外周血单个核细胞对A组链球菌疫苗（菌株J17A4）的增殖反应。在含有1×10(5)个淋巴细胞的培养物中，7天后这种反应达到最大值。38名成年供者中的33名（87%）的细胞产生的刺激指数在2.0至81之间。反应细胞主要是活化辅助性T淋巴细胞所表达的OKT4+、Ia+、Tac+表型。相比之下，12份脐血淋巴细胞样本中有10份未观察到反应。向培养基中添加常规兔抗或单克隆鼠抗Ia（HLA - DR）抗体可消除疫苗诱导的增殖反应。通过有限稀释分析估计的反应细胞频率在每2370个淋巴细胞中有1个至每4580个淋巴细胞中有1个之间。结果表明，A组链球菌疫苗诱导的淋巴细胞刺激代表了一种体外回忆性细胞免疫反应。