Luo Chengchang, Zhang Zunni, Su Qisheng, Mo Wuning
Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang, Autonomous Region, China.
Department of Clinical Laboratory, The People's Hospital of Guangxi Zhuang Autonomous Region & Guangxi Academy of Medical Sciences, Nanning, Guangxi Zhuang, Autonomous Region, China.
Evol Bioinform Online. 2022 Apr 28;18:11769343221095862. doi: 10.1177/11769343221095862. eCollection 2022.
SDF2L1 is a new type of endoplasmic reticulum stress inducible protein, which is related to poor prognosis of various cancer, we initially studied the low expression level of SDF2L1 in NPC, but the molecular mechanism of SDF2L1 in NPC needs further elucidation. To identify phosphorylated proteins regulated by SDF2L1 in nasopharyngeal carcinoma (NPC), Label-free Quantitative (LFQ) Proteomics and 2D-LC-MS/MS analysis were performed on high metastatic NPC 5-8F cells with overexpression of SDF2L1 and empty segment. Western blotting was applied to validate the differentially expressed phosphorylated proteins (DEPPs). As a result, 331 DEPPs were identified by proteomics, and PARVA phosphorylation (ser8) was validated. The present results suggested that PARVA phosphorylation may be a new promising biomarker for predicting NPC and play a key role in the occurrence and development of NPC.
SDF2L1是一种新型的内质网应激诱导蛋白，它与多种癌症的不良预后相关，我们最初研究了SDF2L1在鼻咽癌中的低表达水平，但SDF2L1在鼻咽癌中的分子机制仍需进一步阐明。为了鉴定鼻咽癌（NPC）中受SDF2L1调控的磷酸化蛋白，对过表达SDF2L1和空载体的高转移NPC 5-8F细胞进行了无标记定量（LFQ）蛋白质组学和二维液相色谱-串联质谱（2D-LC-MS/MS）分析。采用蛋白质免疫印迹法验证差异表达的磷酸化蛋白（DEPPs）。结果，通过蛋白质组学鉴定出331种DEPPs，并验证了PARVA的磷酸化（ser8）。目前的结果表明，PARVA磷酸化可能是预测鼻咽癌的一个新的有前景的生物标志物，并在鼻咽癌的发生发展中起关键作用。
