同种免疫性和自身免疫性血小板减少症中的抗血小板调理活性

Anti-platelet opsonic activity in alloimmune and autoimmune thrombocytopenia.

作者信息

Lucas G F, Hadley A G, Holburn A M

出版信息

Clin Lab Haematol. 1987;9(1):59-66. doi: 10.1111/j.1365-2257.1987.tb01382.x.

DOI:10.1111/j.1365-2257.1987.tb01382.x

Abstract

A chemiluminescence technique (CLT) has been developed which measures the interaction between human monocytes and antibody-coated (opsonized) platelets. This technique has an objective end-point, is simple to perform and is of comparable sensitivity to the platelet suspension immunofluorescence test (PSIFT) when used to detect anti-platelet allo-antibodies. In contrast, only 4/20 sera from patients with clinically diagnosed autoimmune thrombocytopenia were opsonic in the CLT, while 8/20 of these same sera bound IgG to platelets in the PSIFT. Only one serum gave positive results in both tests.

摘要

已经开发出一种化学发光技术（CLT），用于测量人类单核细胞与抗体包被（调理）血小板之间的相互作用。该技术有一个客观的终点，操作简单，在用于检测抗血小板同种抗体时，其灵敏度与血小板悬液免疫荧光试验（PSIFT）相当。相比之下，在CLT中，临床诊断为自身免疫性血小板减少症患者的20份血清中只有4份具有调理作用，而在PSIFT中，这些血清中有8份能使IgG与血小板结合。只有一份血清在两种检测中都给出了阳性结果。