Department of Experimental and Clinical Medicine, Faculty of Medicine, Università Politecnica delle Marche, Ancona, Italy.
Department of Medical Biotechnology and Department of Molecular and Developmental Medicine, Obstetrics, and Gynecology, Università Politecnica delle Marche, Ancona, Italy.
F S Sci. 2021 Nov;2(4):407-418. doi: 10.1016/j.xfss.2021.09.002. Epub 2021 Oct 26.
To screen 14 different plant extracts for their antifibrotic effect on human primary leiomyoma and healthy myometrial cells.
Preclinical study.
University research laboratory.
PATIENT(S): Human uterine leiomyoma and matched myometrial tissues were obtained from Caucasian premenopausal women with symptomatic uterine fibroids at the time of hysterectomy.
INTERVENTION(S): Primary human leiomyoma and myometrial cells were cultured in the absence or presence of the plant extracts.
MAIN OUTCOME MEASURE(S): Quantification of the expression of extracellular matrix components, such as fibronectin 1 (FN1), collagen type I alpha 1 (COL1A1), and versican (VCAN), and the profibrotic growth factor activin A or inhibin beta A subunit (INHBA).
RESULT(S): The cells were treated with the 14 extracts for 48 hours, and we measured FN1 messenger RNA (mRNA) expression. Of the 14 extracts, about (ABO) ABO-2 (hop) and ABO-9 (artichoke) significantly reduced FN1 expression in both the cell types. Next, we evaluated the effect of fractions of these 2 extracts on the mRNA expression of FN1 and other extracellular matrix components, such as COL1A1, VCAN, and INHBA, in leiomyoma and myometrial cells. We found that ABO-2 (hop) and ABO-9 (artichoke) as well as their fractions, ABO-AR-2016-015 (fraction of ABO-2) and ABO-AR-2014-168 (fraction of ABO-9), reduced the mRNA expression of FN1, COL1A1, VCAN, and INHBA in primary leiomyoma cells. In primary myometrial cells, the mRNA expression of FN1, COL1A1, VCAN, and INHBA was not greatly affected.
CONCLUSION(S): These results suggest that the hop and artichoke extracts possess antifibrotic properties and support additional evaluation using in vivo models.
筛选 14 种不同植物提取物对人原发性平滑肌瘤和健康子宫平滑肌细胞的抗纤维化作用。
临床前研究。
大学研究实验室。
人子宫肌瘤和匹配的子宫平滑肌组织取自白人绝经前因有症状的子宫肌瘤而行子宫切除术的妇女。
原代人子宫肌瘤和子宫平滑肌细胞在植物提取物存在或不存在的情况下培养。
细胞外基质成分（如纤维连接蛋白 1（FN1）、胶原 I 型 α1（COL1A1）和 versican（VCAN））和促纤维化生长因子激活素 A 或抑制素 B 亚基（INHBA）的表达的定量。
用 14 种提取物处理细胞 48 小时，测量 FN1 信使 RNA（mRNA）表达。在 14 种提取物中，约 ABO-2（啤酒花）和 ABO-9（朝鲜蓟）可显著降低两种细胞类型的 FN1 表达。接下来，我们评估了这两种提取物的分数对 FN1 和其他细胞外基质成分（如 COL1A1、VCAN 和 INHBA）在平滑肌瘤和子宫平滑肌细胞中的 mRNA 表达的影响。我们发现，ABO-2（啤酒花）和 ABO-9（朝鲜蓟）及其分数 ABO-AR-2016-015（ABO-2 的分数）和 ABO-AR-2014-168（ABO-9 的分数）可降低原代平滑肌瘤细胞中 FN1、COL1A1、VCAN 和 INHBA 的 mRNA 表达。在原代子宫平滑肌细胞中，FN1、COL1A1、VCAN 和 INHBA 的 mRNA 表达没有受到很大影响。
这些结果表明，啤酒花和朝鲜蓟提取物具有抗纤维化特性，并支持使用体内模型进行进一步评估。
