Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.
Environ Res. 2022 Sep;212(Pt C):113441. doi: 10.1016/j.envres.2022.113441. Epub 2022 May 11.
Hypochlorite pretreatment has been proven effective in enhancing waste activated sludge (WAS) anaerobic digestion performances recently. In this study, two semi-continuous anaerobic sequencing batch reactors (ASBRs), one fed with Ca(ClO) pretreated thickened WAS (TWAS) and one with raw TWAS, were operated at mesophilic conditions (35 °C) for 145 days. Three loading shocks were introduced to each reactor to compare the performance stability and resilience between the digestion of Ca(ClO) pretreated TWAS and untreated TWAS. Microbial community shifts were quantified to reveal the microbiome responses to disturbances. The results suggested that 1% Ca(ClO) enhanced the digestion of TWAS by inactivating and transforming the biomass to more easily digested substrates. Co-occurrence network analysis revealed that the strongest interactions in the microbial community occurred in the steady state of TWAS anaerobic digestion.
次氯酸盐预处理最近已被证明可有效提高剩余活性污泥（WAS）的厌氧消化性能。在这项研究中，两个半连续厌氧序批式反应器（ASBR），一个用 Ca（ClO）预处理的浓缩剩余活性污泥（TWAS）进料，另一个用原始 TWAS 进料，在中温（35°C）条件下运行了 145 天。每个反应器都引入了三个负荷冲击，以比较 Ca（ClO）预处理 TWAS 和未处理 TWAS 的消化性能稳定性和恢复能力。量化微生物群落变化，揭示微生物组对干扰的反应。结果表明，1%的 Ca（ClO）通过灭活和转化生物量为更易消化的底物来增强 TWAS 的消化。共生网络分析表明，在 TWAS 厌氧消化的稳定状态下，微生物群落中最强的相互作用发生了。
