Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil.
Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil.
J Sep Sci. 2022 Jul;45(14):2478-2487. doi: 10.1002/jssc.202200148. Epub 2022 May 18.
A new method based on Ultraviolet spectrophotometry was developed and compared with that based on high-performance liquid chromatography for the determination and quantification of anthraquinones in the extracts of Rhamnus purshiana bark. A validated quantitative analysis of cascaroside A, cascaroside B, emodin, and aloe-emodin in these herbal products has been previously performed using high-performance liquid chromatography coupled with a diode array detector. In the high-performance liquid chromatography analysis, all the anthraquinones showed satisfactory regression (r > 0.98) within the test ranges, and the recovery was in the range of 94-117%. The limits of detection and quantification were 0.008-0.010 and 0.029-0.035 μg/mL, respectively. Hierarchical cluster analysis and principal component analysis showed differences in the anthraquinones determined from herbal samples. Subsequently, a simple and low-cost ultraviolet spectrophotometric methodology for the quantitative analysis of the same compounds in the extracts was applied, and all the contents were determined. A paired t-test confirmed that there were no significant differences between the two methods. Our results revealed that the developed method is simple and provides the ability to discriminate and control the quality of anthraquinones in herbal products.
建立了一种基于紫外分光光度法的新方法，并将其与高效液相色谱法进行了比较，用于测定和定量分析鼠李皮提取物中的蒽醌类化合物。先前已经使用高效液相色谱法结合二极管阵列检测器对这些草药产品中的 cascaroside A、cascaroside B、大黄素和芦荟大黄素进行了验证定量分析。在高效液相色谱分析中，所有蒽醌类化合物在测试范围内均表现出令人满意的回归（r > 0.98），回收率在 94-117%范围内。检测限和定量限分别为 0.008-0.010 和 0.029-0.035 μg/mL。层次聚类分析和主成分分析显示，从草药样品中测定的蒽醌类化合物存在差异。随后，应用了一种简单且低成本的紫外分光光度法定量分析提取物中相同化合物的方法，并测定了所有含量。配对 t 检验证实两种方法之间没有显着差异。我们的结果表明，所开发的方法简单，并提供了区分和控制草药产品中蒽醌类化合物质量的能力。
