MOA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Institute of Developmental Biology and Neurobiology, Johannes Gutenberg-University, 55128 Mainz, Germany.
Int J Mol Sci. 2022 Apr 20;23(9):4543. doi: 10.3390/ijms23094543.
Movement and fusion of separate cell populations are critical for several developmental processes, such as neural tube closure in vertebrates or embryonic dorsal closure and pupal thorax closure in . Fusion failure results in an opening or groove on the body surface. pupal thorax closure is an established model to investigate the mechanism of tissue closure. Here, we report the identification of T-box transcription factor genes () as Decapentaplegic (Dpp) targets in the leading edge cells of the notum in the late third instar larval and early pupal stages. Reduction of in the notum region results in a thorax closure defect, similar to that in loss-of-function flies. Nine genes are identified as potential downstream targets of Doc in regulating thorax closure by molecular and genetic screens. Our results reveal a novel function of Doc in development. The candidate target genes provide new clues for unravelling the mechanism of collective cell movement.
不同细胞群体的运动和融合对于几个发育过程至关重要，例如脊椎动物神经管闭合或 胚胎背侧闭合和蛹胸闭合。融合失败会导致体表出现开口或凹槽。蛹胸闭合是研究组织闭合机制的成熟模型。在这里，我们报告了 T 盒转录因子基因 () 作为顶盒 (Dpp) 在第三龄幼虫晚期和早期蛹期的翅片前缘细胞中的靶基因的鉴定。在翅片区域减少 会导致胸壁闭合缺陷，类似于 功能丧失的果蝇。通过分子和遗传筛选，确定了九个基因作为 Doc 在调节胸壁闭合中的潜在下游靶基因。我们的结果揭示了 Doc 在 发育中的新功能。候选靶基因提供了揭示细胞集体运动机制的新线索。
