National Engineering Research Center of Tree Breeding and Ecological Remediation, Beijing Forestry University, Beijing 100083, China.
Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2022 Apr 21;23(9):4603. doi: 10.3390/ijms23094603.
After polyploidization, plants usually undergo some morphological and physiological changes, including the lignin content of polyploids usually becoming lower than that of diploids. However, the regulatory mechanism of the variation of lignin content in polyploid plants remains unclear. Therefore, in this research, we used full-sib poplar triploids and diploids to explore the molecular regulatory basis of lignin content in poplar triploid leaves through the determination of lignin content, the observation of xylem cells, and transcriptome sequencing. The results showed that the lignin content of triploid leaves was significantly lower than that of diploid leaves. The xylem cells of triploid leaves were significantly larger than those of diploids. Transcriptome sequencing data show that most lignin biosynthesis genes were significantly downregulated, and genes related to cell growth were mostly upregulated in triploid leaves compared with diploid leaves. In addition, co-expression network analysis showed that several transcription factors might be involved in the regulation of lignin biosynthesis. Consequently, the altered expression of genes related to lignin might lead to the reduced lignin content in triploids. These results provide a theoretical basis for further exploring the molecular mechanism of the variation of polyploid lignin content and the utilization of polyploid lignocellulosic resources.
多倍化后，植物通常会经历一些形态和生理变化，包括多倍体的木质素含量通常低于二倍体。然而，多倍体植物木质素含量变化的调节机制仍不清楚。因此，在这项研究中，我们使用全同胞杨树三倍体和二倍体，通过木质素含量的测定、木质部细胞的观察和转录组测序，来探讨杨树三倍体叶片木质素含量的分子调控基础。结果表明，三倍体叶片的木质素含量显著低于二倍体叶片。三倍体叶片的木质部细胞明显大于二倍体。转录组测序数据表明，与二倍体相比，三倍体叶片中大多数木质素生物合成基因显著下调，与细胞生长相关的基因大多上调。此外，共表达网络分析表明，一些转录因子可能参与了木质素生物合成的调节。因此，与木质素相关的基因表达的改变可能导致三倍体木质素含量的降低。这些结果为进一步探讨多倍体木质素含量变化的分子机制和多倍体木质纤维素资源的利用提供了理论依据。
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