Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland.
Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Norwida 4/6, 50-373 Wroclaw, Poland.
Int J Mol Sci. 2022 Apr 30;23(9):5019. doi: 10.3390/ijms23095019.
Non-coding micro-RNA (miRNAs) regulate the protein expression responsible for cell growth and proliferation. miRNAs also play a role in a cancer cells' response to drug treatment. Knowing that leukemia and lymphoma cells show different responses to active forms of vitamin D, we decided to investigate the role of selected miRNA molecules and regulated proteins, analyzing if there is a correlation between the selected miRNAs and regulated proteins in response to two active forms of vitamin D, calcitriol and tacalcitol. A total of nine human cell lines were analyzed: five leukemias: MV-4-1, Thp-1, HL-60, K562, and KG-1; and four lymphomas: Raji, Daudi, Jurkat, and U2932. We selected five miRNA molecules-miR-27b, miR-32, miR-125b, miR-181a, and miR-181b-and the proteins regulated by these molecules, namely, CYP24A1, Bak1, Bim, p21, p27, p53, and NF-kB. The results showed that the level of selected miRNAs correlates with the level of proteins, especially p27, Bak1, NFκB, and CYP24A1, and miR-27b and miR-125b could be responsible for the anticancer activity of active forms of vitamin D in human leukemia and lymphoma.
非编码微 RNA（miRNA）调节负责细胞生长和增殖的蛋白质表达。miRNA 还在癌细胞对药物治疗的反应中发挥作用。由于知道白血病和淋巴瘤细胞对活性形式的维生素 D 表现出不同的反应，我们决定研究选定的 miRNA 分子和受调控的蛋白质的作用，分析在对两种活性形式的维生素 D，即钙三醇和他卡西醇的反应中，选定的 miRNA 和受调控的蛋白质之间是否存在相关性。总共分析了 9 个人类细胞系：5 种白血病：MV-4-1、Thp-1、HL-60、K562 和 KG-1；4 种淋巴瘤：Raji、Daudi、Jurkat 和 U2932。我们选择了 5 个 miRNA 分子——miR-27b、miR-32、miR-125b、miR-181a 和 miR-181b——以及受这些分子调控的蛋白质，即 CYP24A1、Bak1、Bim、p21、p27、p53 和 NF-kB。结果表明，选定的 miRNA 水平与蛋白质水平相关，特别是 p27、Bak1、NFκB 和 CYP24A1，miR-27b 和 miR-125b 可能负责活性形式的维生素 D 在人类白血病和淋巴瘤中的抗癌活性。
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