Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy.
Istituto Nazionale di Biostrutture e Biosistemi (INBB), 00136 Rome, Italy.
Int J Mol Sci. 2022 May 7;23(9):5219. doi: 10.3390/ijms23095219.
medical device-induced infections affect millions of lives worldwide and innovative preventive strategies are urgently required. Antimicrobial peptides (AMPs) appear as ideal candidates to efficiently functionalize medical devices surfaces and prevent bacterial infections. In this scenario, here, we produced antimicrobial polydimethylsiloxane (PDMS) by loading this polymer with an antimicrobial peptide identified in human apolipoprotein B, r(P)ApoB.
once obtained loaded PDMS, its structure, anti-infective properties, ability to release the peptide, stability, and biocompatibility were evaluated by FTIR spectroscopy, water contact angle measurements, broth microdilution method, time-killing kinetic assays, quartz crystal microbalance analyses, MTT assays, and scanning electron microscopy analyses.
PDMS was loaded with r(P)ApoB peptide which was found to be present not only in the bulk matrix of the polymer but also on its surface. ApoB-derived peptide was found to retain its antimicrobial properties once loaded into PDMS and the antimicrobial material was found to be stable upon storage at 4 °C for a prolonged time interval. A gradual and significant release (70% of the total amount) of the peptide from PDMS was also demonstrated upon 400 min incubation and the antimicrobial material was found to be endowed with anti-adhesive properties and with the ability to prevent biofilm attachment. Furthermore, PDMS loaded with r(P)ApoB peptide was found not to affect the viability of eukaryotic cells.
an easy procedure to functionalize PDMS with r(P)ApoB peptide has been here developed and the obtained functionalized material has been found to be stable, antimicrobial, and biocompatible.
医疗器械相关感染影响了全球数百万人的生命，因此急需创新的预防策略。抗菌肽（AMPs）似乎是有效功能化医疗器械表面并预防细菌感染的理想候选物。在这种情况下，我们通过将载有人载脂蛋白 B 中发现的抗菌肽 r(P)ApoB 的聚合物负载到聚二甲基硅氧烷（PDMS）上来制备抗菌 PDMS。
获得负载 PDMS 后，通过傅里叶变换红外光谱、水接触角测量、肉汤微量稀释法、时效杀菌动力学测定、石英晶体微天平分析、MTT 测定和扫描电子显微镜分析来评估其结构、抗感染性能、释放肽的能力、稳定性和生物相容性。
PDMS 负载 r(P)ApoB 肽，不仅存在于聚合物的基质中，而且存在于其表面。负载到 PDMS 中的 ApoB 衍生肽保留了其抗菌特性，并且在 4°C 下长时间储存时也保持稳定。还证明了肽从 PDMS 中的逐渐和显著释放（总含量的 70%），并且抗菌材料具有抗粘附性和防止生物膜附着的能力。此外，负载 r(P)ApoB 肽的 PDMS 被发现不会影响真核细胞的活力。
这里开发了一种将 r(P)ApoB 肽功能化到 PDMS 的简单方法，并且获得的功能化材料被发现稳定、抗菌且生物相容。
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