Department of Chemical Sciences, University of Naples Federico II, Via Cintia 21, 80126, Naples, Italy.
Department of Chemical Sciences, University of Naples Federico II, Via Cintia 21, 80126, Naples, Italy; Istituto Nazionale di Biostrutture e Biosistemi (INBB), Rome, Italy.
Food Microbiol. 2021 Oct;99:103804. doi: 10.1016/j.fm.2021.103804. Epub 2021 Apr 12.
The effectiveness of three novel "host defence peptides" identified in human Apolipoprotein B (ApoB) as novel antimicrobial and antibiofilm agents to be employed in food industry is reported. ApoB-derived peptides have been found to exert significant antimicrobial effects towards Salmonella typhimurium ATCC® 14028 and Salmonella enteritidis 706 RIVM strains. Furthermore, they have been found to retain antimicrobial activity under experimental conditions selected to simulate those occurring during food storage, transportation and heat treatment, and have been found to be endowed with antibiofilm properties. Based on these findings, to evaluate the applicability of ApoB-derived peptides as food biopreservatives, coating solutions composed by chitosan (CH) and an ApoB-derived peptide have been prepared and found to be able to prevent Salmonella cells attachment to different kinds of surfaces employed in food industry. Finally, obtained coating solution has been demonstrated to hinder microbial proliferation in chicken meat samples. Altogether, obtained findings indicate that ApoB-derived peptides are promising candidates as novel biopreservatives for food packaging.
报告了在人类载脂蛋白 B（ApoB）中鉴定出的三种新型“宿主防御肽”作为新型抗菌和抗生物膜剂在食品工业中应用的有效性。已发现 ApoB 衍生肽对鼠伤寒沙门氏菌 ATCC®14028 和肠炎沙门氏菌 706 RIVM 菌株具有显著的抗菌作用。此外，它们在实验条件下仍保持抗菌活性，这些实验条件是为了模拟食品储存、运输和热处理过程中发生的情况，并且已发现它们具有抗生物膜特性。基于这些发现，为了评估 ApoB 衍生肽作为食品生物防腐剂的适用性，制备了由壳聚糖（CH）和 ApoB 衍生肽组成的涂层溶液，并发现其能够防止沙门氏菌细胞附着在食品工业中使用的不同类型的表面上。最后，所得的涂层溶液被证明可以抑制鸡肉样本中的微生物增殖。总之，研究结果表明，ApoB 衍生肽是作为食品包装新型生物防腐剂的有前途的候选物。
