Dong Xue, Sun Litao, Agarwal Manjree, Maker Garth, Han Yitao, Yu Xiangyang, Ren Yonglin
College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, Perth 6150, Australia.
Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
Foods. 2022 Apr 21;11(9):1211. doi: 10.3390/foods11091211.
Ozone is widely used to control pests in grain and impacts seed germination, a crucial stage in crop establishment which involves metabolic alterations. In this study, dormancy was overcome through after-ripening (AR) in dry barley seed storage of more than 4 weeks; alternatively, a 15-min ozone treatment could break the dormancy of barley immediately after harvest, with accelerated germination efficiency remaining around 96% until 4 weeks. Headspace solid-phase microextraction (HS-SPME) and liquid absorption coupled with gas chromatography mass spectrometry (GC-MS) were utilized for metabolite profiling of 2-, 4- and 7-day germinating seeds. Metabolic changes during barley germination are reflected by time-dependent characteristics. Alcohols, fatty acids, and ketones were major contributors to time-driven changes during germination. In addition, greater fatty acids were released at the early germination stage when subjected to ozone treatment.
臭氧被广泛用于控制谷物中的害虫，且会影响种子萌发，而种子萌发是作物生长的关键阶段，涉及代谢变化。在本研究中，通过对干燥大麦种子进行超过4周的后熟处理来打破休眠；另外，收获后立即用臭氧处理15分钟也可打破大麦休眠，其发芽效率加快，直至4周时仍保持在96%左右。采用顶空固相微萃取（HS-SPME）和液相吸收结合气相色谱-质谱联用（GC-MS）技术对发芽2天、4天和7天的种子进行代谢物分析。大麦萌发过程中的代谢变化呈现出时间依赖性特征。醇类、脂肪酸和酮类是萌发过程中时间驱动变化的主要贡献者。此外，经臭氧处理的种子在萌发早期释放出更多的脂肪酸。
