Alqhtani Abdulmohsen H, Fatemi Seyed A, Elliott Katie E C, Branton Scott L, Evans Jeff D, Leigh Spencer A, Gerard Patrick D, Peebles Edgar D
Department of Poultry Science, Mississippi State University, Starkville, MS 39762, USA.
Department of Animal Production, College of Agriculture and Food Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Animals (Basel). 2022 Apr 27;12(9):1120. doi: 10.3390/ani12091120.
The transmission of the ts-11 strain of (MG) vaccine (ts-11MGV) between incubated eggs and between hatchlings that was administrated via in ovo injection, and its subsequent effects on their posthatch performance were evaluated. Marek's disease diluent alone (sham-injected) or containing either 3.63 × 10, 10, 10, or 10 cfu of ts-11MGV was manually in ovo-injected into the amnion on 18 days of incubation. Egg residue analysis, percentage incubational egg weight loss, hatchability of viable injected eggs, and hatchling body weight (BW) were assessed. Selected hatchlings from each treatment replicate group were swabbed in the choanal cleft for MG DNA detection. Female chick live performance was also assessed through 21 days of posthatch age. Unexposed control sentinel chicks were allocated to each treatment replicate group to assess horizontal transmission. Birds were later swabbed and bled respectively, for detection of MG DNA and IgM production at 21 days posthatch. In all birds, no MG DNA was detected and SPA tests for IgM were negative. Among all variables, only 0 to 21 day BW gain was significantly affected by treatment and was lower in the 3.63 × 10 ts-11 MGV treatment in comparison to all the other treatments. Because ts-11MGV does not exhibit vertical or horizontal transmission capabilities under commercial conditions, it may not be a good candidate for in ovo injection.
评估了通过卵内注射接种的（MG）疫苗ts - 11株（ts - 11MGV）在孵化蛋之间以及雏鸡之间的传播情况，及其对雏鸡出壳后性能的后续影响。在孵化第18天，将单独的马立克氏病稀释液（假注射）或含有3.63×10、10、10或10 cfu ts - 11MGV的稀释液手动卵内注射到羊膜中。评估了卵残留分析、孵化期卵失重百分比、注射后存活卵的孵化率和雏鸡体重（BW）。从每个处理重复组中选取的雏鸡在鼻裂处擦拭以检测MG DNA。还通过雏鸡出壳后21天评估了雌性雏鸡的生长性能。将未接触的对照哨兵雏鸡分配到每个处理重复组以评估水平传播。雏鸡在出壳后21天分别进行擦拭和采血，以检测MG DNA和IgM产生情况。在所有雏鸡中，均未检测到MG DNA，IgM的SPA试验为阴性。在所有变量中，只有0至21天的体重增加受处理显著影响，与所有其他处理相比，3.63×10 ts - 11 MGV处理组的体重增加较低。由于ts - 11MGV在商业条件下不表现出垂直或水平传播能力，它可能不是卵内注射的良好候选疫苗。
