Liu Qin, Zhan Xueyan, Li Dongfang, Zhao Junlong, Wei Haiyong, Alzan Heba, He Lan
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan 430070, China.
Animals (Basel). 2022 May 6;12(9):1197. doi: 10.3390/ani12091197.
is a malaria-like protozoan that parasitizes the red blood cells of canids to cause babesiosis. Due to its high expression and essential function in the survival of parasites, the Glycosylphosphatidylinositol (GPI) anchor protein family is considered an excellent immunodiagnostic marker. Herein, we identified a novel GPI-anchored protein named as BgGPI52-WH with a size of 52 kDa; the recombinant BgGPI52-WH with high antigenicity and immunogenicity was used as a diagnostic antigen to establish a new iELISA method. The iELISA had a sensitivity of 1:400, and no cross-reaction with other apicomplexan parasites occurred. We further demonstrated that the degree of variation was less than 10% using the same samples from the same or different batches of an enzyme-labeled strip. It was found that the method was able to detect early infection (6 days after infection) in the sera of the -infected experimental dogs in which antibody response to rBgGPI52-WH was evaluated. Clinical sera from pet hospitals were further tested, and the average positive rate was about 11.41% (17/149). The results indicate that BgGPI52-WH is a reliable diagnostic antigen, and the new iELISA could be used as a practical method for the early diagnosis of .
是一种类似疟疾的原生动物，寄生于犬科动物的红细胞中导致巴贝斯虫病。由于其在寄生虫存活中的高表达和基本功能，糖基磷脂酰肌醇（GPI）锚定蛋白家族被认为是一种优秀的免疫诊断标志物。在此，我们鉴定了一种名为BgGPI52-WH的新型GPI锚定蛋白，大小为52 kDa；具有高抗原性和免疫原性的重组BgGPI52-WH被用作诊断抗原，建立了一种新的间接ELISA方法。该间接ELISA的灵敏度为1:400，与其他顶复门寄生虫无交叉反应。我们进一步证明，使用来自相同或不同批次酶标条的相同样品，变异程度小于10%。结果发现，该方法能够在评估了对rBgGPI52-WH抗体反应的感染实验犬血清中检测到早期感染（感染后6天）。对宠物医院的临床血清进行了进一步检测，平均阳性率约为11.41%（17/149）。结果表明，BgGPI52-WH是一种可靠的诊断抗原，新的间接ELISA可作为早期诊断的实用方法。
