Yu Xue, Li Zan, Zhao Xinzhe, Hua Liping, Liu Shuanghang, He Changjiu, Yang Liguo, Davis John S, Liang Aixin
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, China.
Animals (Basel). 2022 May 7;12(9):1209. doi: 10.3390/ani12091209.
Anti-Müllerian hormone (AMH) is secreted by the ovaries of female animals and exerts its biological effects through the type II receptor (AMHR2). AMH regulates follicular growth by inhibiting the recruitment of primordial follicles and reducing the sensitivity of antral follicles to FSH. Despite the considerable research on the actions of AMH in granulosa cells, the effect of AMH on the in vitro maturation of oocytes remains largely unknown. In the current study, we showed that AMH is only expressed in cumulus cells, while AMHR2 is produced in both cumulus cells and oocytes. AMH had no significant effect on COCs nuclear maturation, whereas it inhibited the stimulatory effects of FSH on COCs maturation and cumulus expansion. Moreover, AMH treatment effectively inhibited the positive effect of FSH on the mRNA expressions of Hyaluronan synthase 2 (), Pentraxin 3 (), and TNF-alpha-induced protein 6 () genes in COCs. In addition, AMH significantly decreased the FSH-stimulated progesterone production, but did not change estradiol levels. Taken together, our results suggest that AMH may inhibit the effects of FSH-induced COCs in vitro maturation and cumulus expansion. These findings increase our knowledge of the functional role of AMH in regulating folliculogenesis.
抗苗勒管激素（AMH）由雌性动物的卵巢分泌，并通过II型受体（AMHR2）发挥其生物学作用。AMH通过抑制原始卵泡的募集和降低窦状卵泡对促卵泡生成素（FSH）的敏感性来调节卵泡生长。尽管对AMH在颗粒细胞中的作用进行了大量研究，但AMH对卵母细胞体外成熟的影响仍 largely未知。在本研究中，我们发现AMH仅在卵丘细胞中表达，而AMHR2在卵丘细胞和卵母细胞中均有产生。AMH对卵丘-卵母细胞复合体（COCs）的核成熟没有显著影响，而它抑制了FSH对COCs成熟和卵丘扩展的刺激作用。此外，AMH处理有效抑制了FSH对COCs中透明质酸合酶2（）、五聚素3（）和肿瘤坏死因子α诱导蛋白6（）基因mRNA表达的积极影响。另外，AMH显著降低了FSH刺激的孕酮产生，但没有改变雌二醇水平。综上所述，我们的结果表明AMH可能抑制FSH诱导的COCs体外成熟和卵丘扩展的作用。这些发现增加了我们对AMH在调节卵泡发生中的功能作用的认识。
