肝脏受体同源物-1通过孕激素受体信号通路调控牛卵巢颗粒细胞凋亡。
Liver Receptor homolog-1 Regulates Apoptosis of Bovine Ovarian Granulosa Cells by Progestogen Receptor Signaling Pathway.
作者信息
Xu Dejun, Jiang Xiaohan, Wang Yukun, Song Shuaifei
机构信息
Chongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing 400715, China.
College of Animal Science and Technology, Northwest A&F University, No. 3 Taicheng Road, Xianyang 712100, China.
出版信息
Animals (Basel). 2022 May 8;12(9):1213. doi: 10.3390/ani12091213.
The purpose of the present investigation was to assess the function of LRH-1 on GCs and the mechanisms involved. Here, LRH- was highly expressed in the bovine GCs of atretic follicles. Treatment with 50 μM of LRH-1 agonist (DLPC) significantly induced the expression of LRH-1 (p < 0.05). In particular, LRH-1 activation blocked the progestogen receptor signaling pathway via downregulating progesterone production and progestogen receptor levels (p < 0.05), but had no effect on 17 beta-estradiol synthesis. Meanwhile, LRH-1 activation promoted the apoptosis of GCs and increased the activity of caspase 3 (p < 0.05). Importantly, upregulating the progestogen receptor signaling pathway with progestogen could attenuate the LRH-1-induced proapoptotic effect. Moreover, treatment with progestogen decreased the activity of the proapoptotic gene caspase 3 and increased the expression of antiapoptotic gene Bcl2 in LRH-1 activated GCs (p < 0.05). Taken together, these results demonstrate that LRH-1 might be dependent on the progestogen receptor signaling pathway to modulate bovine follicular atresia.
本研究的目的是评估肝受体同源物-1（LRH-1）在颗粒细胞（GCs）中的功能及其相关机制。在此，LRH-1在闭锁卵泡的牛颗粒细胞中高表达。用50μM的LRH-1激动剂（二肉豆蔻酰磷脂酰胆碱，DLPC）处理显著诱导了LRH-1的表达（p<0.05）。特别地，LRH-1激活通过下调孕酮生成和孕激素受体水平阻断了孕激素受体信号通路（p<0.05），但对17β-雌二醇合成没有影响。同时，LRH-1激活促进了颗粒细胞的凋亡并增加了半胱天冬酶3的活性（p<0.05）。重要的是，用孕激素上调孕激素受体信号通路可减弱LRH-1诱导的促凋亡作用。此外，孕激素处理降低了LRH-1激活的颗粒细胞中促凋亡基因半胱天冬酶3的活性并增加了抗凋亡基因Bcl2的表达（p<0.05）。综上所述，这些结果表明LRH-1可能依赖于孕激素受体信号通路来调节牛卵泡闭锁。
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