Kien Truong Quy, Toan Pham Quoc, Nghia Phan Ba, Van Diem Thi, Duc Nguyen Van, Ha Do Manh, Dung Nguyen Thi Thuy, Ha Nguyen Thi Thu, Quyen Le Thi Bao, Vinh Hoang Trung, Manh Bui Van, Su Hoang Xuan, Tien Tran Viet, Thang Le Viet, Rostaing Lionel
Department of Nephrology, Military Hospital 103, Vietnam Military Medical University, Hanoi 100000, Vietnam.
Department of Microbiology, Faculty of Biology, University of Science, National University of Hanoi, Hanoi 100000, Vietnam.
J Clin Med. 2022 May 1;11(9):2544. doi: 10.3390/jcm11092544.
The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region.
344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to investigate the number of BKV infections. Positive samples with a sufficient virus concentration were analyzed by nested PCR in the VP1 region, sequencing detected genotyping and single-nucleotide polymorphism.
BKV infection was determined in 214 patients (62.2%), of whom 11 (5.1%) were diagnosed with BKV-associated nephropathy. Among the 90 BKV-I strains sequenced, 89 (98.88%) were strains of I/b-1 and 1 (1.12%) was strain I/b-2. The 60 BKV-IV strains had a greater diversity of subgroups, including 40% IV/a-1, 1.66% IV/a-2, 56.68% IV/c-1, and 1.16% IV/c-2. Additionally, of 11 cases diagnosed with BKVN, seven belonged to subgroup I/b-1 (63.6%) and four to subgroup IV/c-1 (36.4%). Moreover, 22 specific SNPs that were genotype I or IV were determined in this Vietnamese population. Specifically, at position 1745, for the Vietnamese BKV-IV strains, the SNP position (A→G) appeared in 57/60 samples (95%). This causes transformation of the amino acid N→S. This SNP site can enable detection of genotype IV in Vietnam. It represents a unique evolution pattern and mutation that has not been found in other international strains.
The BKV-I genotype was more common than BKV-IV; however, mutations that occur on the VP1 typing region of BKV-IV strains were more frequent than in BKV-I strains.
本研究的目的是基于VP1基因区域鉴定肾移植越南受者中循环的SNP位点并确定BK病毒（BKV）基因型。
从越南第103军事医院的肾移植受者中收集344份样本，以调查BKV感染的数量。对病毒浓度足够的阳性样本在VP1区域进行巢式PCR分析，测序检测基因分型和单核苷酸多态性。
214例患者（62.2%）被确定感染BKV，其中11例（5.1%）被诊断为BKV相关性肾病。在测序的90株BKV-I毒株中，89株（98.88%）为I/b-1毒株，1株（1.12%）为I/b-2毒株。60株BKV-IV毒株的亚组多样性更大，包括40%的IV/a-1、1.66%的IV/a-2、56.68%的IV/c-1和1.16%的IV/c-2。此外，在11例诊断为BKVN的病例中，7例属于I/b-1亚组（63.6%），4例属于IV/c-1亚组（36.4%）。此外，在该越南人群中确定了22个I型或IV型特异性SNP。具体而言，在第1745位，对于越南BKV-IV毒株，SNP位点（A→G）出现在57/60个样本中（95%）。这导致氨基酸N→S的转变。该SNP位点可用于在越南检测IV型基因型。它代表了一种独特的进化模式和突变，在其他国际毒株中未发现。
BKV-I基因型比BKV-IV更常见；然而，BKV-IV毒株VP1分型区域发生的突变比BKV-I毒株更频繁。
