Nissan Nour, Hooker Julia, Pattang Arezo, Charette Martin, Morrison Malcolm, Yu Kangfu, Hou Anfu, Golshani Ashkan, Molnar Stephen J, Cober Elroy R, Samanfar Bahram
Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON K1A 0C6, Canada.
Department of Biology, Ottawa Institute of Systems Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.
Plants (Basel). 2022 Apr 24;11(9):1146. doi: 10.3390/plants11091146.
Soybean is a valuable crop, used in animal feed and for human consumption. Selecting soybean cultivars with low seed cadmium (Cd) concentration is important for the purpose of minimizing the transfer of Cd into the human body. To ensure international trade, farmers need to produce soybean that meets the European Union (EU) Cd limit of 0.2 mg kg. In this study, we evaluated two populations of recombinant inbred lines (RILs), X5154 and X4050, for seed Cd accumulation. Linkage maps were constructed with 325 and 280 polymorphic simple sequence repeat (SSR) markers, respectively, and used to identify a novel minor quantitative trait locus (QTL) on chromosome 13 in the X4050 population between SSR markers Satt522 and Satt218. Based on a gene ontology search within the QTL region, seven genes were identified as candidates responsible for low seed Cd accumulation, including and . In addition, we confirmed the known major gene, , in the X5154 population and developed KASP and CAPS/dCAPS allele-specific markers for efficient marker-assisted breeding for .
大豆是一种重要的作物，用于动物饲料和人类消费。选择种子镉（Cd）浓度低的大豆品种对于尽量减少镉向人体的转移至关重要。为确保国际贸易，农民需要生产符合欧盟镉限量0.2毫克/千克的大豆。在本研究中，我们评估了两个重组自交系（RIL）群体X5154和X4050的种子镉积累情况。分别用325和280个多态性简单序列重复（SSR）标记构建连锁图谱，并用于在X4050群体中位于SSR标记Satt522和Satt218之间的13号染色体上鉴定一个新的微效数量性状位点（QTL）。基于在QTL区域内的基因本体搜索，鉴定出7个基因作为负责低种子镉积累的候选基因，包括 和 。此外，我们在X5154群体中确认了已知的主基因 ，并开发了KASP和CAPS/dCAPS等位基因特异性标记，用于 的高效标记辅助育种。
