Department of Respiratory Medicine, Ningde Municipal Hospital of Ningde Normal University, Ningde, 352100, People's Republic of China.
J Cardiothorac Surg. 2022 May 14;17(1):117. doi: 10.1186/s13019-022-01861-3.
MicroRNAs have been confirmed as vital regulators in gene expression, which could affect multiple cancer cell biological behaviors. This study aims to elucidate the molecular mechanism of miR-144-3p in lung cancer cellular proliferation and metastasis.
MiR-144-3p expression in lung cancer tissues and cell lines was detected by qRT-PCR. HGF was predicted as the target gene of miR-144-3p using TargetScan and dual luciferase reporter assay. Immunohistochemistry and qRT-PCR were used to explore the impacts of HCF on lung cancer tissues and cell lines. Impacts of miR-144-3p and HGF on cancer cellular proliferation, migration and invasion were elucidated by CCK-8, Flow cytometry, Transwell invasion and Wound-healing assay. Moreover, nude mouse xenograft model was established to evaluate the effects of miR-144-3p on lung cancer cells.
MiR-144-3p exhibited a reduction in both lung cancer tissues and cell lines. HGF was a direct target of miR-144-3p. In contrast to the miR-144-3p expression level, HGF showed a higher level in lung cancer tissues and cell lines. Overexpression miR-144-3p suppressed A549 and NCI-H1299 cell proliferation and metastasis, whereas this was reversed by HGF. MiR-144-3p exhibited an inhibitory effect on A549 cell-induced tumor growth of nude mice.
This study reveals miR-144-3p/HGF axis may be involved in the suppression of lung cancer cellular proliferation and development, and miR-144-3p may function as a potential therapeutic target in lung cancer treatment in the future.
microRNAs 已被证实为基因表达的重要调节因子，可影响多种癌细胞的生物学行为。本研究旨在阐明 miR-144-3p 在肺癌细胞增殖和转移中的分子机制。
通过 qRT-PCR 检测肺癌组织和细胞系中的 miR-144-3p 表达。使用 TargetScan 和双荧光素酶报告基因检测预测 HGF 是 miR-144-3p 的靶基因。免疫组化和 qRT-PCR 用于探讨 HCF 对肺癌组织和细胞系的影响。通过 CCK-8、流式细胞术、Transwell 侵袭和划痕愈合实验探讨 miR-144-3p 和 HGF 对癌细胞增殖、迁移和侵袭的影响。此外，建立裸鼠异种移植模型评估 miR-144-3p 对肺癌细胞的影响。
miR-144-3p 在肺癌组织和细胞系中均呈现下调表达。HGF 是 miR-144-3p 的直接靶基因。与 miR-144-3p 的表达水平相反，HGF 在肺癌组织和细胞系中呈现高表达。过表达 miR-144-3p 抑制了 A549 和 NCI-H1299 细胞的增殖和转移，而 HGF 的过表达则逆转了这一现象。miR-144-3p 对 A549 细胞诱导的裸鼠肿瘤生长具有抑制作用。
本研究揭示了 miR-144-3p/HGF 轴可能参与抑制肺癌细胞的增殖和发展，miR-144-3p 可能成为未来肺癌治疗的潜在治疗靶点。
