Salehi Mohammad, Darroudi Majid, Musavi Maryam, Momtazi-Borojeni Amir Abbas
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Medical Biotechnology, Nuclear Medicine, Research Center Mashhad University of Medical Sciences, Mashhad, Iran.
Curr Stem Cell Res Ther. 2025;20(4):464-477. doi: 10.2174/011574888X291147240507072107.
Aging is a phenomenon which occurs over time and leads to the decay of living organisms. During the progression of aging, some age-associated diseases including cardiovascular disease, cancers, and neurological, mental, and physical disorders could develop. Genetic and epigenetic factors like microRNAs, as one of the post-transcriptional regulators of genes, play important roles in senescence. The self-renewal and differentiation capacity of mesenchymal stem cells makes them good candidates for regenerative medicine.
The objective of this study is to evaluate senescence-related miRNAs in human MSCs using bioinformatics analysis.
In this study, the Gene Expression Omnibus (GEO) database was used to investigate the senescence-related genome profile. Then, down-regulated genes were selected for further bioinformatics analysis with the assumption that their decreased expression is associated with an increased aging process. Considering that miRNAs can interfere in gene expression, miRNAs complementary to these genes were identified using bioinformatics software.
Through bioinformatics analysis, we predicted hsa-miR-590-3p, hsa-miR-10b-3p, hsamiR- 548 family, hsa-miR-144-3p, and hsa-miR-30b-5p which involve in cellular senescence and the aging of human MSCs.
miRNA mimics or anti-miRNA agents have the potential to be used as anti-aging tools for MSCs.
衰老 是一种随时间发生的现象，会导致生物体的衰退。在衰老过程中，可能会出现一些与年龄相关的疾病，包括心血管疾病、癌症以及神经、精神和身体紊乱。基因和表观遗传因素，如作为基因转录后调节因子之一的微小RNA（microRNAs），在衰老过程中发挥着重要作用。间充质干细胞的自我更新和分化能力使其成为再生医学的良好候选者。
本研究的目的是通过生物信息学分析评估人骨髓间充质干细胞中与衰老相关的微小RNA。
在本研究中，使用基因表达综合数据库（Gene Expression Omnibus，GEO）来研究与衰老相关的基因组概况。然后，选择下调基因进行进一步的生物信息学分析，假设它们表达的降低与衰老过程的增加有关。考虑到微小RNA可以干扰基因表达，使用生物信息学软件鉴定与这些基因互补的微小RNA。
通过生物信息学分析，我们预测了参与细胞衰老和人骨髓间充质干细胞衰老的hsa-miR-590-3p、hsa-miR-10b-3p、hsamiR-548家族、hsa-miR-144-3p和hsa-miR-30b-5p。
微小RNA模拟物或抗微小RNA剂有潜力用作骨髓间充质干细胞的抗衰老工具。
