Ferreira Santos Mauro S, Kurfman Emily, Zamuruyev Konstantin, Noell Aaron C, Mora Maria F, Willis Peter A
Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California, USA.
Department of Chemistry, University of Kansas, Lawrence, Kansas, USA.
Electrophoresis. 2023 Jan;44(1-2):10-14. doi: 10.1002/elps.202200085. Epub 2022 Jun 3.
Capillary electrophoresis (CE) systems have undergone extensive development for spaceflight applications. A flight-compatible high voltage power supply and the necessary voltage isolation for other energized components can be large contributors to both the volume and mass of a CE system, especially if typical high voltage levels of 25-30 kV are used. Here, we took advantage of our custom CE hardware to perform a trade study for simultaneous optimization of capillary length, high voltage level, and separation time, without sacrificing method performance. A capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C D) method recently developed by our group to target inorganic cations and amino acids relevant to astrobiology was used as a test case. The results indicate that a 50 cm long capillary with 15 kV applied voltage (half of that used in the original method) can be used to achieve measurement goals while minimizing instrument size.
毛细管电泳（CE）系统在航天应用方面已经历了广泛的发展。一个与飞行兼容的高压电源以及为其他通电部件所需的电压隔离，可能会在很大程度上增加CE系统的体积和质量，特别是如果使用25 - 30 kV的典型高压水平时。在此，我们利用定制的CE硬件进行了一项权衡研究，以在不牺牲方法性能的情况下，同时优化毛细管长度、高压水平和分离时间。我们小组最近开发的一种用于检测与天体生物学相关的无机阳离子和氨基酸的带有电容耦合无接触电导检测的毛细管电泳（CE - CD）方法被用作测试案例。结果表明，使用一根50厘米长的毛细管，施加15 kV电压（原始方法中使用电压的一半），可以在最小化仪器尺寸的同时实现测量目标。
