富氢水诱导的气孔关闭依赖于拟南芥中的 GPA1。

Stomatal closure induced by hydrogen-rich water is dependent on GPA1 in Arabidopsis thaliana.

机构信息

Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500, Kunming, China.

Foshan Institute of Agricultural Science, Foshan, 528145, China.

出版信息

Plant Physiol Biochem. 2022 Jul 15;183:72-75. doi: 10.1016/j.plaphy.2022.04.015. Epub 2022 Apr 20.

DOI:10.1016/j.plaphy.2022.04.015

PMID:35569167

Abstract

Hydrogen (H) is a new signaling molecule that regulates stomatal closure via stimulating the generation of reactive oxygen species (ROS) and nitric oxide (NO) in Arabidopsis thaliana. GPA1 is the sole heterotrimeric G protein canonical α subunit found in Arabidopsis genome and functions in stomatal closure. Here, we estimated a possible role of Arabidopsis GPA1 in hydrogen-rich water (HRW)-induced stomatal closure. Our data indicated that HRW induced significant stomatal closure as well as the generation of ROS and NO in the Col-0 guard cells. However, the production of ROS and NO and stomatal closure induced by HRW were absent in the gpa1-4 mutant lacking the expression of AtGPA1. By contrast, overexpression of AtGPA1 in gpa1-4 (AtGPA1-HA/gpa1-4) restored stomatal closure and the generation of NO and ROS in the presence of HRW. Taken together, our results suggest that GPA1 is necessary for HRW-induced stomatal closure in Arabidopsis.

摘要

氢气（H）是一种新的信号分子，通过刺激拟南芥中活性氧（ROS）和一氧化氮（NO）的产生来调节气孔关闭。GPA1 是拟南芥基因组中唯一发现的异三聚体 G 蛋白经典α亚基，在气孔关闭中起作用。在这里，我们估计了拟南芥 GPA1 在富氢水中（HRW）诱导气孔关闭中的可能作用。我们的数据表明，HRW 诱导 Col-0 保卫细胞中显著的气孔关闭以及 ROS 和 NO 的产生。然而，在缺乏 AtGPA1 表达的 gpa1-4 突变体中，HRW 诱导的 ROS 和 NO 的产生以及气孔关闭是不存在的。相比之下，在 gpa1-4 中过表达 AtGPA1（AtGPA1-HA/gpa1-4）在 HRW 存在的情况下恢复了气孔关闭和 NO 和 ROS 的产生。综上所述，我们的结果表明，GPA1 是 HRW 诱导拟南芥气孔关闭所必需的。