School of Water Conservancy and Hydroelectric Power, Hebei University of Engineering, Handan, 056038, China; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, 3010, Australia.
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, 300191, China.
J Environ Manage. 2022 Aug 15;316:115264. doi: 10.1016/j.jenvman.2022.115264. Epub 2022 May 12.
Total solid content (TS) is an important factor for biogas production during anaerobic digestion. In this study, we explored the influence of different TS (5% wet, 15% semi-solid and 25% solid state) on the relative cumulative methane production (RCMP) during anaerobic co-digestion of chicken manure with corn straw. Results showed that total ammonium nitrogen and free ammonia nitrogen concentration increased with the increase of TS. Ammonium nitrogen in treatments at 15% TS was 2.25-2.76 times as high as that at 5% TS, which was below 3 times. The highest chemical oxygen demand removal and RCMP were obtained in the treatment of 15% TS with a ratio of 2:1 chicken manure: corn straw (based on TS). The RCMP in the treatments of 15% TS were 3.63-4.59 times higher than that of 5% TS based on the volume of substrates. The abundance of Caldicoprobacter improving the degradation of corn straw was significantly positively correlated with the RCMP, and the average abundance of Caldicoprobacter at 15% TS was 8.33 and 7.02 times higher than that at 5% and 25% TS, respectively. Structural equation models analysis suggested that TS significantly impacted the RCMP by indirectly impacting free ammonia nitrogen and microbial abundance. These findings indicated semi-solid state (15% TS) decreased ammonia nitrogen releasing and improved the abundance of Caldicoprobacter, and increased RCMP during anaerobic co-digestion of chicken manure with corn straw.
总固体含量（TS）是厌氧消化过程中产沼气的一个重要因素。在本研究中，我们探讨了不同 TS（5%湿、15%半固体和 25%固体）对鸡粪与玉米秸秆厌氧共消化过程中相对累积甲烷产量（RCMP）的影响。结果表明，总铵氮和游离氨氮浓度随 TS 的增加而增加。在 15%TS 处理中，铵氮是 5%TS 的 2.25-2.76 倍，低于 3 倍。在鸡粪：玉米秸秆（基于 TS）比为 2:1 的 15%TS 处理中，获得了最高的化学需氧量去除率和 RCMP。15%TS 处理的 RCMP 分别是 5%TS 和 25%TS 处理的 3.63-4.59 倍。提高玉米秸秆降解的卡尔迪克普罗布acter 的丰度与 RCMP 呈显著正相关，15%TS 下卡尔迪克普罗布acter 的平均丰度分别比 5%TS 和 25%TS 高 8.33 倍和 7.02 倍。结构方程模型分析表明，TS 通过间接影响游离氨氮和微生物丰度对 RCMP 产生显著影响。这些发现表明，半固体状态（15%TS）降低了氨氮的释放，提高了卡尔迪克普罗布acter 的丰度，从而增加了鸡粪与玉米秸秆厌氧共消化过程中的 RCMP。
