Soybean Research Institute & MARA National Center for Soybean Improvement & MARA Key Laboratory of Biology and Genetic Improvement of Soybean (General) & State Key Laboratory for Crop Genetics and Germplasm Enhancement & Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Soybean Research Institute & MARA National Center for Soybean Improvement & MARA Key Laboratory of Biology and Genetic Improvement of Soybean (General) & State Key Laboratory for Crop Genetics and Germplasm Enhancement & Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Genomics. 2022 Jul;114(4):110387. doi: 10.1016/j.ygeno.2022.110387. Epub 2022 May 13.
Soybean mosaic virus (SMV) strain SC11 was prevalent in middle China. Its resistance was controlled by a Mendelian single dominant gene R in soybean Kefeng-1. This study aimed at mapping R and identifying its candidate gene. R locus was mapped ~217 kb interval between two SNP-linkage-disequilibrium-blocks (Gm02_BLOCK_11273955_11464884 and Gm02_BLOCK_11486875_11491354) in W82.a1.v1 genome using recombinant inbred lines population derived from Kefeng-1 (Resistant) × NN1138-2 (Susceptible), but inserted with a ~245 kb segment in W82.a2.v1 genome. In the entire 462 kb R region, 429 SNPs, 142 InDels and 34 putative genes were identified with more SNPs/InDels distributed in non-functional regions. Thereinto, ten genes contained SNP/InDel variants with high and moderate functional impacts on proteins, among which Glyma.02G119700 encoded a typical innate immune receptor-like kinase involving in virus disease process and responded to SMV inoculation, therefore was recognized as R's candidate gene. The novel R locus and candidate genes may help developing SMV resistance germplasm.
大豆花叶病毒（SMV）SC11 株在中国中部流行。其抗性由大豆科丰 1 中的一个孟德尔显性单基因 R 控制。本研究旨在定位 R 并鉴定其候选基因。利用科丰 1（抗性）×NN1138-2（敏感）衍生的重组自交系群体，在 W82.a1.v1 基因组中两个 SNP 连锁不平衡块（Gm02_BLOCK_11273955_11464884 和 Gm02_BLOCK_11486875_11491354）之间将 R 位点定位到217 kb 的区间，但在 W82.a2.v1 基因组中插入了一个245 kb 的片段。在整个 462 kb 的 R 区域中，鉴定出了 429 个 SNPs、142 个 InDels 和 34 个推定基因，非功能区域分布着更多的 SNPs/InDels。其中，有 10 个基因包含对蛋白质具有高和中度功能影响的 SNP/InDel 变体，其中 Glyma.02G119700 编码一个典型的先天免疫受体样激酶，参与病毒发病过程并对 SMV 接种产生反应，因此被认为是 R 的候选基因。该新型 R 基因座和候选基因可能有助于开发 SMV 抗性种质资源。
