National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, The Key Laboratory of Crop Genetics and Breeding of Hebei, Shijiazhuang 050035, China.
Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, The Key Laboratory of Crop Genetics and Breeding of Hebei, Shijiazhuang 050035, China.
Plant Sci. 2020 Mar;292:110367. doi: 10.1016/j.plantsci.2019.110367. Epub 2019 Dec 5.
Soybean mosaic virus (SMV) is a major pathogen causing yield loss. Developing soybean plants tolerant or resistant to SMV is important for mitigating the adverse effects of the viral infection. However, most studies have focused on the resistance to normal SMV strains. Thus, investigations of the resistance or tolerance to the novel recombinant SMV strain have been limited. To address the threat of the recombinant SMV, two soybean parent genotypes with contrasting reactions to the recombinant SMV and 211 F recombinant inbred lines were evaluated under artificial inoculation conditions. The JD12 plants are resistant to the recombinant SMV, whereas HT is highly tolerant, but still susceptible. Genetic analyses suggested that the resistance of JD12 is controlled by a single dominant gene and the tolerance is a quantitative trait. The QTL mapping results revealed one QTL (qTsmv-13) for resistance and two QTLs (qTsmv-2 and qTsmv-3) for tolerance. A comparison between known resistance genes and the QTLs identified in this study suggested that qTsmv-13 and qTsmv-2 may correspond to Rsv1 and Rsv4, respectively, whereas qTsmv-3 represents a newly identified QTL for SMV tolerance. We further delimited qTsmv-3 to an interval of approximately 86 kb with a map-based cloning strategy. Only two of five candidate genes, Glyma.03G00550 and Glyma.03G00570, varied between the parents. Additionally, Glyma.03G00550, which is a multidrug and toxic compound extrusion transporter gene, is the likely candidate gene for qTsmv-3. In summary, our research opens a new avenue for formulating strategies to breed soybean varieties tolerant to SMV.
大豆花叶病毒（SMV）是导致产量损失的主要病原体。培育对 SMV 具有耐受性或抗性的大豆品种对于减轻病毒感染的不利影响非常重要。然而，大多数研究都集中在对普通 SMV 株系的抗性上。因此，对新型重组 SMV 株系的抗性或耐受性的研究有限。为了应对重组 SMV 的威胁，在人工接种条件下，对两种对重组 SMV 反应不同的大豆亲本基因型和 211 个重组自交系进行了评估。JD12 植株对重组 SMV 具有抗性，而 HT 则高度耐受，但仍易感。遗传分析表明，JD12 的抗性由单个显性基因控制，而耐受性是一个数量性状。QTL 定位结果揭示了一个抗性 QTL（qTsmv-13）和两个耐病性 QTL（qTsmv-2 和 qTsmv-3）。将本研究中鉴定的已知抗性基因与 QTL 进行比较表明，qTsmv-13 和 qTsmv-2 可能分别对应于 Rsv1 和 Rsv4，而 qTsmv-3 代表一个新发现的 SMV 耐病性 QTL。我们进一步利用基于图谱的克隆策略将 qTsmv-3 限定在约 86kb 的区间内。在父母本之间，只有五个候选基因中的两个，Glyma.03G00550 和 Glyma.03G00570 存在差异。此外，Glyma.03G00550 是一个多药和有毒化合物外排转运蛋白基因，是 qTsmv-3 的候选基因。综上所述，我们的研究为制定培育对 SMV 具有耐受性的大豆品种的策略开辟了新途径。
