College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei, 230036, China.
College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei, 230036, China; Anhui Province Key Laboratory of Local Livestock, Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China.
Fish Shellfish Immunol. 2022 Jun;125:212-219. doi: 10.1016/j.fsi.2022.05.006. Epub 2022 May 13.
Normal dissolved oxygen in water is essential for maintaining the physiological functions of fish, but environmental pollution, such as eutrophication can lead to a decrease in oxygen content in water. How this reduction of dissolved oxygen in water affects the immune functions of fish and the potential regulatory mechanisms have not been thoroughly elucidated. In this study, we made full use of the aquatic model animal zebrafish to explore this question. In a model of LPS-induced inflammation, we found that hypoxia induced by infusing nitrogen into water increased the expression of pro-inflammatory cytokines, such as il-1β, il-6, and il-8. In vivo imaging also showed that hypoxia significantly increased neutrophil migration to the site of caudal fin injury in the transgenic line. Subsequently, we found that the phosphorylation level of ERK protein was significantly activated upon hypoxia and proved the roles of ERK signaling in the expression of pro-inflammatory cytokines and neutrophil migration in zebrafish. This study indicated that reduced water oxygen significantly increases the inflammatory response of the zebrafish.
水中正常的溶解氧对维持鱼类的生理功能至关重要，但环境污染，如富营养化，会导致水中的氧气含量下降。水中溶解氧的减少如何影响鱼类的免疫功能以及潜在的调节机制尚未被彻底阐明。在这项研究中，我们充分利用水生模式动物斑马鱼来探讨这个问题。在 LPS 诱导的炎症模型中，我们发现通过向水中注入氮气引起的缺氧会增加促炎细胞因子如 il-1β、il-6 和 il-8 的表达。体内成像也显示缺氧显著增加了转基因系中尾部 fin 损伤部位中性粒细胞的迁移。随后，我们发现 ERK 蛋白的磷酸化水平在缺氧时显著激活，并证明了 ERK 信号在斑马鱼中促炎细胞因子表达和中性粒细胞迁移中的作用。这项研究表明，水中氧气减少会显著增加斑马鱼的炎症反应。
