Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control/College of Animal Science, Tarim University, Alar, Xinjiang 843300, China.
Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, QG, Egypt.
Curr Drug Deliv. 2023;20(9):1327-1336. doi: 10.2174/1567201819666220513121219.
The development of nanogels has become an attractive strategy to enhance the antibacterial activity performance of bacteria.
The ciprofloxacin composite nanogels were successfully prepared by electrostatic interaction between gelatin (positive charge) and CMC (negative charge) with the help of sodium tripolyphosphate (TPP) as ionic crosslinkers, to increase the antibacterial activity of ciprofloxacin against Staphylococcus aureus (S. aureus) mastitis infection. The formulation screening, characterization, in vitro release, antibacterial activity, and biosafety were studied.
The optimized formulation was fabricated of 20 mg/mL (CMC) and 50mg/mL (gelatin). The optimized ciprofloxacin composite nanogels were homogenous canary yellow suspension with a sedimentation rate of 1 and were incorporated in nano-sized cross-linked polymeric networks. The particle sizes were distributed as, 402.7±1.3 nm, PDI of 0.12±0.01, ZP of -24.5±0.2mv, EE of 74.28%±0.03%, LC of 20.5%±0.05%. Scanning electron microscope images revealed that ciprofloxacin might be incorporated in nano-sized cross-linked polymeric networks. Fourier transform infrared showed that the spontaneous electrostatic interactions between CMC and gelatin produce the network structure and form the composite nanogels. Meanwhile, in vitro release study showed that ciprofloxacin composite nanogels had sustained-release performances. The ciprofloxacin composite nanogels had shown better antibacterial activity against SCV 102 isolate than S. aureus ATCC 29213 and S. aureus 101isolates. The biosafety studies suggested the great promise of the injectable ciprofloxacin composite nanogels as a biocompatible breast injection.
This study will afford a potential approach for developing injectable ciprofloxacin-loaded gelatin-CMC composite nanogels for cow S. aureus mastitis therapy.
纳米凝胶的发展已成为提高细菌抗菌活性的一种有吸引力的策略。
通过静电相互作用，利用三聚磷酸钠（TPP）作为离子交联剂，成功制备了载环丙沙星复合纳米凝胶，以提高环丙沙星对金黄色葡萄球菌（金黄色葡萄球菌）乳腺炎感染的抗菌活性。研究了配方筛选、表征、体外释放、抗菌活性和生物安全性。
优化的配方由 20mg/ml（CMC）和 50mg/ml（明胶）组成。优化的载环丙沙星复合纳米凝胶为均匀的浅黄色悬浮液，沉降率为 1，掺入纳米交联聚合物网络中。粒径分布为 402.7±1.3nm，PDI 为 0.12±0.01，ZP 为-24.5±0.2mv，EE 为 74.28%±0.03%，LC 为 20.5%±0.05%。扫描电子显微镜图像显示，环丙沙星可能被掺入纳米交联聚合物网络中。傅里叶变换红外光谱显示，CMC 和明胶之间的自发静电相互作用产生了网络结构并形成了复合纳米凝胶。同时，体外释放研究表明，载环丙沙星复合纳米凝胶具有缓释性能。载环丙沙星复合纳米凝胶对 SCV102 分离株的抗菌活性优于金黄色葡萄球菌 ATCC29213 和金黄色葡萄球菌 101 分离株。生物安全性研究表明，可注射载环丙沙星明胶-CMC 复合纳米凝胶作为一种生物相容性乳房注射具有很大的应用前景。
本研究为开发用于治疗奶牛金黄色葡萄球菌乳腺炎的可注射载环丙沙星明胶-CMC 复合纳米凝胶提供了一种潜在的方法。
