蜘蛛蟹（Hyas araneus）幼体发育过程中的蜕皮甾类水平。

Ecdysteroid levels during the larval development of the spider crab Hyas araneus.

作者信息

Spindler K D, Anger K

出版信息

Gen Comp Endocrinol. 1986 Oct;64(1):122-8. doi: 10.1016/0016-6480(86)90037-7.

Abstract

Ecdysteroid levels were determined by radioimmunoassay during the larval development of the spider crab Hyas araneus L. In each of the three larval instars (zoea I, zoea II, megalopa) a minimum in ecdysteroid levels was found during early postmoult and a maximum during premoult. The amounts of ecdysteroids increased during development and reached maxima of 93, 137, and 165 pg ecdysone equivalents per larva in zoea I, zoea II, and megalopa, respectively. Ecdysteroid concentrations per unit of body dry weight or carbon increased during each moulting cycle, but the average values showed decreasing tendency from the first to the last instar. The main ecdysteroid in all larval instars and during the moulting cycle was 20-OH-ecdysone, and practically no ecdysone could be detected by HPLC-RIA.

摘要

采用放射免疫分析法测定了蜘蛛蟹（Hyas araneus L.）幼体发育过程中的蜕皮甾类水平。在三个幼体期（第一期蚤状幼体、第二期蚤状幼体、大眼幼体）的每一个阶段，蜕皮甾类水平在蜕皮后早期出现最低值，在蜕皮前出现最高值。蜕皮甾类的含量在发育过程中增加，在第一期蚤状幼体、第二期蚤状幼体和大眼幼体中，每只幼体的蜕皮甾酮当量分别达到93、137和165 pg的最大值。在每个蜕皮周期中，单位体干重或碳中的蜕皮甾类浓度增加，但平均值从第一期到最后一期呈下降趋势。在所有幼体期和蜕皮周期中，主要的蜕皮甾类是20-羟基蜕皮酮，通过高效液相色谱-放射免疫分析法几乎检测不到蜕皮酮。