School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
ACS Macro Lett. 2022 May 17;11(5):636-642. doi: 10.1021/acsmacrolett.2c00095. Epub 2022 Apr 20.
Biodegradable and lipid-like highly branched poly(β-amino ester)s, HPAESA, were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. Biodegradability reduces the cytotoxicity of HPAESA and enables controlled DNA release. Lipid mimicry enhances cellular uptake and endosomal escape of HPAESA/DNA polyplexes. HPAESA are able to transfect rat adipose-derived stem cells (rADSs) and human ADSCs (hADSCs) with orders of magnitude higher efficiency than commercial gene transfection reagents, with cell viability exceeding 90%. Most importantly, HPAESA can effectively transfer the nerve growth factor (NGF)-encoding plasmid to rADSCs and induce high NGF secretion, which significantly promotes neurite outgrowth of PC12 cells.
可生物降解和类脂的高度支化的聚（β-氨基酯），HPAESA，被开发出来通过基因转染来增强脂肪来源干细胞的生物学功能。生物降解性降低了 HPAESA 的细胞毒性，并实现了 DNA 的控制释放。类脂模拟增强了 HPAESA/DNA 超分子复合物的细胞摄取和内涵体逃逸。HPAESA 能够以比商业基因转染试剂高几个数量级的效率转染大鼠脂肪来源干细胞（rADSs）和人脂肪来源干细胞（hADSCs），细胞活力超过 90%。最重要的是，HPAESA 可以有效地将神经生长因子（NGF）编码质粒转移到 rADSCs 中，并诱导高 NGF 分泌，这显著促进 PC12 细胞的突起生长。
