Townsend D S, Moger W H
Horm Behav. 1987 Mar;21(1):93-9. doi: 10.1016/0018-506x(87)90034-1.
Males of the Puerto Rican frog Eleutherodactylus coqui practice parental care of terrestrial eggs throughout embryonic development (17-26 days). Parental care is associated with marked changes in male behavior, including the cessation of normal calling activity (necessary for mate attraction) and reduction of egg cannibalism. To analyze the relationship between parental behavior and androgens, blood was collected from male frogs in the field and plasma analyzed for total androgens. Parental males had significantly lower androgen levels than both nonparental, calling males, and amplectant (mating) males. The decline in circulating androgens between the sexually active state (calling and mating) and the parental care state was fairly rapid (less than 12 hr). Androgen levels did not differ among parental males at different stages of the parental care period.
波多黎各蛙类“寇奎雨蛙”的雄性个体在整个胚胎发育过程（17 - 26天）中对陆地产卵进行亲代抚育。亲代抚育与雄性行为的显著变化有关，包括正常鸣叫活动（吸引配偶所必需）的停止以及卵同类相食行为的减少。为了分析亲代行为与雄激素之间的关系，在野外从雄性蛙类采集血液，并分析血浆中的总雄激素含量。进行亲代抚育的雄性个体的雄激素水平显著低于未进行亲代抚育的鸣叫雄性个体以及抱对（交配）雄性个体。从性活跃状态（鸣叫和交配）到亲代抚育状态，循环雄激素水平的下降相当迅速（不到12小时）。在亲代抚育期不同阶段的亲代雄性个体中，雄激素水平没有差异。
