Integrative Muscle Physiology Laboratory, Appalachian State University; Department of Health and Exercise Science, Appalachian State University.
Integrative Muscle Physiology Laboratory, Appalachian State University; Department of Health and Exercise Science, Appalachian State University; Department of Biology, Appalachian State University;
J Vis Exp. 2022 Apr 28(182). doi: 10.3791/63933.
Previously developed rodent resistance-based exercise models, including synergistic ablation, electrical stimulation, weighted-ladder climbing, and most recently, weighted-sled pulling, are highly effective at providing a hypertrophic stimulus to induce skeletal muscle adaptations. While these models have proven invaluable for skeletal muscle research, they are either invasive or involuntary and labor-intensive. Fortunately, many rodent strains voluntarily run long distances when given access to a running wheel. Loaded wheel running (LWR) models in rodents are capable of inducing adaptations commonly observed with resistance training in humans, such as increased muscle mass and fiber hypertrophy, as well as stimulation of muscle protein synthesis. However, the addition of moderate wheel load either fails to deter mice from running great distances, which is more reflective of an endurance/resistance training model, or the mice discontinue running nearly entirely due to the method of load application. Therefore, a novel high-load wheel running model (HLWR) has been developed for mice where external resistance is applied and progressively increased, enabling mice to continue running with much higher loads than previously utilized. Preliminary results from this novel HLWR model suggest it provides sufficient stimulus to induce hypertrophic adaptations over the 9 week training protocol. Herein, the specific procedures to execute this simple yet inexpensive progressive resistance-based exercise training model in mice are described.
先前开发的基于啮齿动物抗阻的运动模型，包括协同消融、电刺激、负重梯攀爬，以及最近的负重雪橇拉动，都非常有效地提供了一个肥大刺激，以诱导骨骼肌适应。虽然这些模型已被证明对骨骼肌研究非常有价值，但它们要么具有侵入性，要么是不自主的、劳动强度大。幸运的是，许多啮齿动物在被允许使用跑步轮时会自愿跑很远的距离。啮齿动物的负重轮跑（LWR）模型能够诱导与人类抗阻训练中常见的适应，如肌肉质量和纤维肥大增加，以及肌肉蛋白合成的刺激。然而，适度的轮载增加要么不能阻止老鼠跑很远的距离，这更能反映出耐力/抗阻训练模型，要么老鼠由于加载方式而几乎完全停止跑步。因此，已经为老鼠开发了一种新的高负荷轮跑模型（HLWR），其中施加并逐渐增加外部阻力，使老鼠能够以比以前更高的负荷继续跑步。该新型 HLWR 模型的初步结果表明，它在 9 周的训练方案中提供了足够的刺激，以诱导肥大适应。本文描述了在小鼠中执行这种简单但廉价的渐进式抗阻运动训练模型的具体步骤。
