Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Hemmat highway, next to Milad tower, Tehran, Iran.
Arch Microbiol. 2022 May 16;204(6):327. doi: 10.1007/s00203-022-02914-w.
Recent studies have established the possible role of microbiota in developing various diseases. In this regard, attention has shifted to the evaluation of microbiota changes in the paranasal sinuses and its relationship to chronic rhinosinusitis (CRS), especially CRS with nasal polyposis (CRSwNP). This study aimed to examine the bacterial communities of the sphenoidal sinus in Iranian patients with and without CRS. The investigation included 36 subjects, including 18 patients with CRSwNP who underwent Functional Endoscopic Sinus Surgery (FESS) and 18 non-CRS patients who underwent Endoscopic Endonasal Approach (EEA) for pituitary adenoma. The surgeries were performed under general anesthesia, and the sphenoidal sinus was sampled using sterile rayon-tipped swabs coated with a sheet. TaqMan quantitative real-time polymerase chain reaction (qPCR) method (the 16S rDNA gene from bacteria) was used for detection of bacterial communities in different samples. Staphylococcus haemolyticus and Pseudomonas aeruginosa were significantly more prevalent in CRS patients than non-CRS patients (P value ≤ 0.05). However, no significant difference in the frequency of Corynebacterium spp. and Staphylococcus aureus was observed between the two groups, and no Streptococcus pneumoniae or Haemophilus influenza species were isolated from any of the samples. The current study's findings indicated a significant difference in the frequency of certain bacterial species in patients with CRS vs. non-CRS patients. By establishing a link between microbial burden and CRS, it is possible to develop effective treatments or even prevent disorders in this body area.
最近的研究已经确定了微生物群在多种疾病发展中的可能作用。在这方面，人们的注意力已经转移到评估鼻窦中的微生物群变化及其与慢性鼻-鼻窦炎（CRS），特别是伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNP）的关系上。本研究旨在检查伊朗患有和不患有 CRS 的患者的蝶窦细菌群落。该调查包括 36 名受试者，其中 18 名 CRSwNP 患者接受了功能性内窥镜鼻窦手术（FESS），18 名非 CRS 患者因垂体腺瘤接受了内窥镜经鼻入路（EEA）。手术在全身麻醉下进行，使用无菌的粘胶拭子从蝶窦取样，拭子上涂有一张薄片。使用 TaqMan 定量实时聚合酶链反应（qPCR）方法（来自细菌的 16S rDNA 基因）检测不同样本中的细菌群落。与非 CRS 患者相比，CRS 患者的溶血葡萄球菌和绿脓假单胞菌明显更为常见（P 值≤0.05）。然而，两组之间棒状杆菌和金黄色葡萄球菌的频率没有显著差异，也没有从任何样本中分离出肺炎链球菌或流感嗜血杆菌。本研究的结果表明，CRS 患者与非 CRS 患者的某些细菌种类的频率存在显著差异。通过建立微生物负荷与 CRS 之间的联系，有可能开发出有效的治疗方法，甚至可以预防该身体部位的疾病。
