UMR Animal, Santé, Territoires, Risque et Ecosystèmes (ASTRE), CIRAD-INRAE-Université de Montpellier, Montpellier, France.
Laboratoire de Ploufragan/Plouzané/Niort, ANSES, Ploufragan, France.
Methods Mol Biol. 2022;2503:105-118. doi: 10.1007/978-1-0716-2333-6_7.
Molecular biology methods are highly sensitive to detect the genome of pathogens and to study their biology. Polymerase chain reaction (PCR) and reverse transcription followed by a polymerase chain reaction (RT-PCR) permit the detection of the presence and the replication of African swine fever virus in soft ticks. Here, we described our techniques to detect and quantify DNA and RNA of African swine fever virus in soft ticks including a housekeeping gene of soft ticks as internal control.
分子生物学方法高度敏感，可用于检测病原体的基因组并研究其生物学特性。聚合酶链反应（PCR）和逆转录聚合酶链反应（RT-PCR）可用于检测软蜱中非洲猪瘟病毒的存在和复制。在此，我们描述了用于检测和定量软蜱中非洲猪瘟病毒 DNA 和 RNA 的技术，包括软蜱管家基因作为内参。
