Department of Biological Sciences and Museum of Natural Science, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Biological Sciences and Museum of Natural Science, Louisiana State University, Baton Rouge, LA 70803, USA.
Mol Phylogenet Evol. 2022 Aug;173:107525. doi: 10.1016/j.ympev.2022.107525. Epub 2022 May 14.
Although recent molecular phylogenetic analyses of Lepidothrix manakins (family Pipridae) have helped clarify their evolutionary relationships, the placement of several lineages remains in question because of low or conflicting branch support. In particular, the relationship of L. coronata to other members of the genus and relationships within the L. nattereri + L. vilasboasi + L. iris clade have been difficult to resolve. We used RADcap to collect restriction site-associated DNA sequence data and estimate the first subspecies-level phylogeny of the genus Lepidothrix (17 of 18 currently recognized subspecies), and we included extensive geographic representation of the widespread and phenotypically variable L. coronata. We found strong support for the phylogenetic position and monophyly of L. coronata, and we resolved two clades separated by the Andes that, along with previous divergence time estimates and our assessment of morphological and vocal evidence, suggest the presence of two biological species: Velvety Manakin (L. velutina) west of the Andes and Blue-capped Manakin (L. coronata) east of the Andes. Species-level relationships within the L. nattereri + L. vilasboasi + L. iris clade remained poorly resolved in concatenated and coalescent-based analyses, with SNAPP analyses suggesting that the lack of reciprocal monophyly is due to extensive allele sharing among these taxa. Finally, we confirmed a previously documented hybrid between L. coronata and L. suavissima as an F1 individual, consistent with the view that hybridization between these two species is a rare event and that postmating reproductive barriers prevent successful backcrossing.
虽然最近对 Lepidothrix 蜂虎（蜂虎科）的分子系统发育分析有助于阐明它们的进化关系，但由于分支支持率低或存在冲突，一些谱系的位置仍然存在疑问。特别是，Lepidothrix coronata 与该属其他成员的关系以及 L. nattereri + L. vilasboasi + L. iris 分支内的关系一直难以解决。我们使用 RADcap 收集了限制位点相关的 DNA 序列数据，并估计了 Lepidothrix 属的第一个亚种水平的系统发育（包括目前公认的 18 个亚种中的 17 个），并广泛代表了广泛分布且表型多变的 L. coronata。我们发现 L. coronata 的系统发育位置和单系性得到了强有力的支持，并且我们解决了两个由安第斯山脉隔开的分支，加上之前的分化时间估计和我们对形态和发声证据的评估，表明存在两个生物物种：安第斯山脉以西的绒蜂虎（L. velutina）和安第斯山脉以东的蓝顶蜂虎（L. coronata）。在联合和基于合并的分析中，L. nattereri + L. vilasboasi + L. iris 分支内的种间关系仍然没有很好地解决，SNAPP 分析表明，缺乏相互单系性是由于这些类群之间广泛的等位基因共享。最后，我们证实了之前记录的 L. coronata 和 L. suavissima 之间的杂交是一个 F1 个体，这与这两个物种之间的杂交是一个罕见事件的观点一致，并且交配后生殖障碍阻止了成功的回交。
