鉴定 KLF14 三级结构及其在脑肿瘤进展中的诊断意义。
Determining KLF14 tertiary structure and diagnostic significance in brain cancer progression.
机构信息
Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.
Minneapolis VA Health Care System Research Service, Minneapolis, MN, USA.
出版信息
Sci Rep. 2022 May 16;12(1):8039. doi: 10.1038/s41598-022-12072-0.
Expression analysis of new protein targets may play a crucial role in the early detection and diagnosis of brain tumor progression. The study aimed to investigate the possible relation of KLF14, TPD52, miR-124, and PKCε in the development and progression of brain cancer and space occupying lesion (SOL) of the brain. One hundred human blood samples comprising varying diagnostic groups (SOL brain, grade I, II, III, IV) were analyzed by real-time quantitative PCR to determine the expression level of KLF14, TPD52, miR-124, and PKCε. TPD52 and PKCε were upregulated in brain cancer by 2.5- and 1.6-fold, respectively, whereas, KLF14 and miR-124 were downregulated in brain cancer. In metastatic and high-grade brain cancer, TPD52 and PKCε expression were up-regulated and KLF14 and miR-124 expression were down-regulated. Further, these genes were found to be differentially expressed in the blood of patients with SOL. Upregulation of TPD52 and PKCε, however, reduced expression of KLF14 and miR-124 in SOL of the brain as compared to healthy controls. Expression analysis of TPD52, KLF14, miR-124, and PKCε provided useful information on the differences existing between the normal brain and SOL, in addition to gliomas; thus, might prove to be useful having diagnostic or prognostic value.
新蛋白靶标的表达分析可能在脑肿瘤进展的早期检测和诊断中发挥关键作用。本研究旨在探讨 KLF14、TPD52、miR-124 和 PKCε 在脑癌和脑占位性病变（SOL）发展和进展中的可能关系。通过实时定量 PCR 分析 100 个人血样本，这些样本包括不同的诊断组（SOL 脑、I 级、II 级、III 级、IV 级），以确定 KLF14、TPD52、miR-124 和 PKCε 的表达水平。TPD52 和 PKCε 在脑癌中分别上调了 2.5 倍和 1.6 倍，而 KLF14 和 miR-124 在脑癌中下调。在转移性和高级别脑癌中，TPD52 和 PKCε 的表达上调，KLF14 和 miR-124 的表达下调。此外，这些基因在 SOL 患者的血液中表现出差异表达。与健康对照组相比，TPD52 和 PKCε 的上调导致 KLF14 和 miR-124 在脑 SOL 中的表达降低。TPD52、KLF14、miR-124 和 PKCε 的表达分析提供了关于正常大脑和 SOL 之间存在差异的有用信息，除了神经胶质瘤；因此，可能具有诊断或预后价值。
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