miR-139-5p 通过靶向 TPD52 抑制胃癌的发展。
MiR-139-5p Inhibits the Development of Gastric Cancer through Targeting TPD52.
机构信息
The Department of Emergency Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Neuroelectro Physiological Laboratory, People's Hospital of Linzi District, Zibo, Shandong Province, China.
出版信息
J Healthc Eng. 2022 Feb 16;2022:4033373. doi: 10.1155/2022/4033373. eCollection 2022.
BACKGROUND
Many researchers have confirmed that miRNAs are involved in the pathogenesis of gastric cancer (GC). This study focused on investigating the specific functions of miR-139-5p in GC.
METHODS
MiR-139-5p and TPD52 expressions were observed by qRT-PCR or western blot in GC. The functional mechanism of miR-139-5p was explored by the luciferase reporter assay, transwell assay, and MTT assay.
RESULTS
MiR-139-5p downregulation and TPD52 upregulation were detected in GC. Adverse clinical features and prognosis in GC patients were related to low miR-139-5p expression. MiR-139-5p overexpression restrained GC cell proliferation and metastasis. Furthermore, miR-139-5p directly targeted TPD52. TPD52 silencing blocked GC progression. And TPD52 upregulation weakened the antitumor effect of miR-139-5p in GC.
CONCLUSION
MiR-139-5p inhibits GC cell proliferation and metastasis through downregulating TPD52.
背景
许多研究人员已经证实，miRNAs 参与了胃癌（GC）的发病机制。本研究专注于研究 miR-139-5p 在 GC 中的具体功能。
方法
通过 qRT-PCR 或 Western blot 观察 GC 中 miR-139-5p 和 TPD52 的表达。通过荧光素酶报告基因检测、transwell 检测和 MTT 检测探索 miR-139-5p 的功能机制。
结果
在 GC 中检测到 miR-139-5p 的下调和 TPD52 的上调。GC 患者的不良临床特征和预后与低 miR-139-5p 表达有关。miR-139-5p 的过表达抑制了 GC 细胞的增殖和转移。此外，miR-139-5p 可以直接靶向 TPD52。TPD52 的沉默阻断了 GC 的进展。并且 TPD52 的上调削弱了 miR-139-5p 在 GC 中的抗肿瘤作用。
结论
miR-139-5p 通过下调 TPD52 抑制 GC 细胞的增殖和转移。
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