C1QTNF6 受 miR-184 靶向调控，调节肺腺癌细胞的增殖、迁移和侵袭。
C1QTNF6 Targeted by MiR-184 Regulates the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells.
机构信息
Department of Cardio-Thoracic Surgery, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, No. 365 Renming East Road, Wucheng District, Jinhua, 321000, Zhejiang, China.
出版信息
Mol Biotechnol. 2022 Nov;64(11):1279-1287. doi: 10.1007/s12033-022-00495-z. Epub 2022 May 17.
OBJECTIVE
To seek out the mechanism by which C1QTNF6 mediates lung adenocarcinoma (LUAD).
METHODS
Differentially expressed mRNAs and miRNAs in LUAD were analyzed using bioinformatics. In LUAD cells, C1QTNF6 mRNA and miR-184 expression were evaluated with qRT-PCR, and C1QTNF6 protein level was assessed by western blot. Cellular behaviors were assessed by colony formation, CCK-8, Transwell, and wound healing methods. The binding ability of miR-184 to C1QTNF6 was observed by dual-luciferase assay.
RESULTS
High expression of C1QTNF6 in LUAD stimulated cancer cellular behaviors. MiR-184 was lowly expressed in LUAD and downregulated C1QTNF6 expression. MiR-184 restrained LUAD cell processes by targeting C1QTNF6.
CONCLUSION
MiR-184 repressed LUAD cell processes via mediating C1QTNF6. MiR-184 and C1QTNF6 are expected to be indicators for LUAD treatment.
目的
探索 C1QTNF6 介导肺腺癌（LUAD）的机制。
方法
采用生物信息学方法分析 LUAD 中差异表达的 mRNA 和 miRNA。用 qRT-PCR 检测 LUAD 细胞中 C1QTNF6 mRNA 和 miR-184 的表达，用 Western blot 检测 C1QTNF6 蛋白水平。用集落形成、CCK-8、Transwell 和划痕愈合方法评估细胞行为。通过双荧光素酶报告基因实验观察 miR-184 与 C1QTNF6 的结合能力。
结果
在 LUAD 中 C1QTNF6 的高表达刺激了癌症细胞行为。miR-184 在 LUAD 中低表达，下调 C1QTNF6 的表达。miR-184 通过靶向 C1QTNF6 抑制 LUAD 细胞进程。
结论
miR-184 通过介导 C1QTNF6 抑制 LUAD 细胞进程。miR-184 和 C1QTNF6 有望成为 LUAD 治疗的指标。
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