Citrus Research International, PO Box 2201, Matieland 7602, South Africa; Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Citrus Research International, PO Box 2201, Matieland 7602, South Africa; Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
J Virol Methods. 2022 Aug;306:114543. doi: 10.1016/j.jviromet.2022.114543. Epub 2022 May 14.
Plum viroid I (PlVd-I) is found in marbling and corky flesh diseased plum trees in South Africa. In this study a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the high-throughput detection of PlVd-I was developed. This assay can be performed on crude extracts and detection can either be a pH dependent colorimetric reaction or a real-time fluorescent signal reaction. The false discovery rate was shown to be low and no decrease in sensitivity was detected compared to RT-PCR. The RT-LAMP assay allows for the fast and cost-effective detection of PlVd-I that will curtail the distribution of infected plant material.
李痘病毒 I（PlVd-I）存在于南非患有大理石纹和木栓化果肉的李树中。本研究开发了一种用于 PlVd-I 高通量检测的逆转录环介导等温扩增（RT-LAMP）检测方法。该检测方法可在粗提物上进行，检测可以是依赖 pH 的比色反应，也可以是实时荧光信号反应。结果表明假阳性率较低，与 RT-PCR 相比，检测灵敏度没有降低。RT-LAMP 检测方法可快速、经济有效地检测 PlVd-I，从而遏制受感染植物材料的传播。
