用于兽医血液学血液分析的多通道血细胞计数系统的改进与评估

J Am Vet Med Assoc. 1987 Feb 15;190(4):411-5.

A multichannel, semiautomated, blood cell counting system (Coulter Counter Model S550) was modified for use in veterinary hematology by increasing both the erythrocyte and leukocyte aperture currents to 225 V and 195 V, respectively, followed by calibration with human blood. It was evaluated by use of 350 samples from dogs, cats, horses, and cows. Values for leukocyte count, erythrocyte count, mean corpuscular volume, and hematocrit generated by the S550 were compared with values generated by an automated multichannel counter with histogram capability and other reference procedures when appropriate. Mean differences for values between S550 and reference values were less than calibration tolerance limits for the instrument. Correlation coefficients were excellent for all values of each species. To assess behavior of leukocytes of the different species with respect to the counting threshold, leukocyte size distribution histograms were generated for all samples analyzed on the S550. Means for mean leukocyte volumes in diluent and lysing reagents were 55.5, 56.6, 67.4, and 72.8 fl for dogs, cats, horses, and cows, respectively. Canine leukocyte counts, because of small leukocyte size, were an average of 14% less for 5 samples analyzed on the unmodified instrument, compared with analysis after increasing the leukocyte aperture current. Leukocyte threshold failures attributable to interfering particles, resulting in falsely high counts, were recognized in 14%, 10%, 8% and 0% of feline, bovine, canine, and equine samples, respectively. The magnitude of error in these samples averaged 5% for cows and dogs, but was considered not important. However, leukocyte counts of feline samples in this group averaged 44% falsely high.

一种多通道、半自动血细胞计数系统（库尔特计数器S550型）经改装后用于兽医血液学，方法是将红细胞和白细胞孔径电流分别增加到225 V和195 V，然后用人血进行校准。使用来自犬、猫、马和牛的350份样本对其进行评估。将S550生成的白细胞计数、红细胞计数、平均红细胞体积和血细胞比容值与具有直方图功能的自动多通道计数器生成的值以及适当情况下的其他参考程序生成的值进行比较。S550与参考值之间的平均差异小于仪器的校准公差限度。每个物种所有值的相关系数都非常好。为了评估不同物种白细胞相对于计数阈值的行为，为在S550上分析的所有样本生成了白细胞大小分布直方图。犬、猫、马和牛在稀释剂和溶血试剂中的平均白细胞体积分别为55.5、56.6、67.4和72.8飞升。由于白细胞体积小，与增加白细胞孔径电流后进行分析相比，在未改装的仪器上分析的5份犬样本的白细胞计数平均低14%。在猫、牛、犬和马的样本中，分别有14%、10%、8%和0%的样本出现了因干扰颗粒导致白细胞阈值失败，从而导致计数错误偏高。这些样本中的误差幅度在牛和犬中平均为5%，但被认为不太重要。然而，该组中猫样本的白细胞计数平均错误偏高44%。