Weiser M G
Department of Pathology, Colorado State University, Fort Collins.
Vet Pathol. 1987 Nov;24(6):560-3. doi: 10.1177/030098588702400615.
Using a single channel electronic cell counter and attached particle size analyzer, leukocyte size distribution histograms were determined on canine, feline, bovine, and equine blood diluted with chloride-based diluent and treated with a conventional stromatolysin. Histograms were usually unimodal, but a few were bimodal. Mean values for mean lysed leukocyte particle volume were 49.2, 51.1, 55.4, and 65.0 fl for canine, feline, equine, and bovine blood, respectively. From inspection of histograms, a lower threshold of 30 fl referenced to latex spheres was interpreted to be appropriate for counting leukocytes of these four species simultaneously. Debris below the threshold was seen in many samples and was usually separated from the leukocyte population by a valley touching the histogram baseline at the threshold channel. Debris resulted in a visually detectable threshold failure by extending considerably into the leukocyte size range in 9% of feline, 9% of canine, and 7% of bovine samples. It is recommended that careful establishment of the lower counting threshold will minimize frequency and severity of leukocyte count error associated with failure to exclude debris.
使用单通道电子细胞计数器和附带的粒度分析仪，对用氯化物基稀释剂稀释并用传统溶基质素处理的犬、猫、牛和马的血液进行白细胞大小分布直方图测定。直方图通常为单峰，但也有少数为双峰。犬、猫、马和牛血液的平均裂解白细胞颗粒体积的平均值分别为49.2、51.1、55.4和65.0飞升。通过检查直方图，相对于乳胶球，30飞升的较低阈值被认为适合同时计数这四个物种的白细胞。在许多样本中都可见低于该阈值的碎片，并且通常通过在阈值通道处与直方图基线接触的谷将其与白细胞群体分开。在9%的猫样本、9%的犬样本和7%的牛样本中，碎片通过大量延伸到白细胞大小范围内导致视觉上可检测到的阈值失败。建议仔细确定较低的计数阈值，以尽量减少与未能排除碎片相关的白细胞计数误差的频率和严重程度。
