Fuchimukai T, Fujiwara T, Takahashi A, Enhorning G
J Appl Physiol (1985). 1987 Feb;62(2):429-37. doi: 10.1152/jappl.1987.62.2.429.
With a pulsating bubble surfactometer we assessed the ability of various agents, fibrinogen, human serum, albumin, and a 55,000-dalton serum protein, to interfere with the surface activity of Surfactant TA. From a highest final protein concentration of 4 mg/ml the potential inhibitors were diluted down to 0.125 mg/ml in six steps, and each concentration was evaluated together with two final phospholipid concentrations, 6.25 and 1.25 mg/ml, of the surfactant preparation. The strongest inhibiting action was exerted by fibrinogen, followed by human serum and the 55,000-dalton serum protein; the weakest inhibitor was albumin. Bilirubin, when added in an amount of 1.73 mg/100 ml dissolved in human serum, significantly (P less than 0.001) augmented the inhibition over that exerted by human serum alone. Adsorption rate, as reflected in the mean value of surface tension 2 and 10 s after creation of a bubble, not pulsating, was seriously affected by each of the protein-containing inhibitors in concentrations exceeding 1 mg/ml. Surface tension was raised significantly when the pulsating bubble was at maximal and minimal size. The effect was dose dependent. At maximal size it showed no tendency to disappear during the 10-min recording, but at minimal bubble size the inhibition gradually diminished. We conclude that proteins present in the airways may seriously interfere with the activity of Surfactant TA.
我们使用脉动气泡表面张力测定仪评估了多种物质（纤维蛋白原、人血清、白蛋白以及一种55000道尔顿的血清蛋白）干扰表面活性剂TA表面活性的能力。从最高终浓度4mg/ml开始，将潜在抑制剂分六步稀释至0.125mg/ml，每种浓度与表面活性剂制剂的两种终磷脂浓度（6.25mg/ml和1.25mg/ml）一起进行评估。纤维蛋白原的抑制作用最强，其次是人血清和55000道尔顿的血清蛋白；最弱的抑制剂是白蛋白。当以1.73mg/100ml的量溶解于人血清中加入胆红素时，与单独的人血清相比，其抑制作用显著增强（P＜0.001）。在产生非脉动气泡后2秒和10秒时表面张力的平均值所反映的吸附速率，受到每种浓度超过1mg/ml的含蛋白质抑制剂的严重影响。当脉动气泡处于最大和最小尺寸时，表面张力显著升高。这种影响呈剂量依赖性。在最大尺寸时，在10分钟的记录过程中没有消失的趋势，但在最小气泡尺寸时，抑制作用逐渐减弱。我们得出结论，气道中存在的蛋白质可能会严重干扰表面活性剂TA的活性。
