School of Life Sciences, University of Warwick, Coventry, UK.
Warwick Medical School, University of Warwick, Coventry, UK.
Methods Mol Biol. 2022;2479:11-19. doi: 10.1007/978-1-0716-2233-9_2.
We present a recombineering-based method for editing the genome of a temperate phage. The method uses the lambda Red recombination system to edit the genome of a lysogenized host with a prophage compatible with bacteriophage lambda. Linear DNA is used as the recombination substrate and antibiotic resistance is used as the basis for selection of recombinants. The method enables the genetic manipulation of a prophage in 3-5 days.
我们提出了一种基于重组的方法来编辑温和噬菌体的基因组。该方法利用 λ Red 重组系统，以与噬菌体 λ 相容的前噬菌体为基础，编辑溶原化宿主的基因组。线性 DNA 用作重组底物，抗生素抗性用作选择重组体的基础。该方法可在 3-5 天内完成前噬菌体的基因操作。
