Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Jozef Stefan Institute, Ljubljana, Slovenia.
Methods Mol Biol. 2022;2447:233-246. doi: 10.1007/978-1-0716-2079-3_19.
In plants, the response to stress, such as salinity, pathogen attack, drought, high concentration of metals, hyperthermia, and hypothermia, is usually accompanied by potassium ion (K) leakage from the cytosol to the cell wall, mediated by plasma membrane cation conductivity. Stress-induced electrolyte leakage co-occurs with accumulation of reactive oxygen species (ROS) and calcium ions (Ca) and often results in programmed cell death (PCD). The development of X-ray and mass spectrometry (MS) based imaging techniques has enabled insight into the spatial tissue and cell-specific redistribution of major and trace elements during the stress response. In this chapter a workflow for sample preparation, imaging, and image analysis by X-ray and MS based techniques is presented.
在植物中，对胁迫（如盐度、病原体攻击、干旱、高浓度金属、高温和低温）的反应通常伴随着钾离子（K）从细胞质漏到细胞壁，这是由质膜阳离子电导介导的。胁迫诱导的电解质渗漏与活性氧（ROS）和钙离子（Ca）的积累同时发生，并且通常导致程序性细胞死亡（PCD）。基于 X 射线和质谱（MS）的成像技术的发展使人们能够深入了解在应激反应过程中主要和微量元素在空间组织和细胞特异性的再分配。在本章中，介绍了一种基于 X 射线和 MS 的技术进行样品制备、成像和图像分析的工作流程。
