Fujisawa S, Kadoma Y, Masuhara E
J Biomed Mater Res. 1987 Jan;21(1):89-98. doi: 10.1002/jbm.820210112.
The interaction of 22 lipid-soluble small molecules, widely used in dental materials and devices, with synthetic phospholipid liposomes, was investigated by the application of differential scanning calorimetry (DSC), in order to clarify the mechanism of small molecules in biological systems. Dimyristoyl phosphatidyl choline (DMPC) and dipalmitoyl phosphatidyl choline (DPPC) were used as lipids. The interaction of various monomers with liposomes has been reported by us in this journal. In this experiment, various compounds such as redox-initiator, photo-initiator or photo-sensitizer, inhibitor, root-canal disinfectant, cement-base materials (eugenol and 2-ethoxybenzoic acid) etc., were investigated. From changes in the phase transition temperature (T), enthalpy (delta H kcal mol-1) and H/HHW values of both DMPC and DPPC liposomes induced by these molecules, it was concluded that aromatic tertiary amine, benzoyl peroxide and hydroquinone monomethyl ether had a relatively large effect on liposomes, and that in phenol derivatives, m-cresol and p-chlorophenol had a large effect on liposomes compared to phenol. Eugenol used as dental cement also had a large effect on liposomes due to its high hydrophobicity. Changes in the transition properties of liposomes induced by small molecules (characterized by shift of T to a lower temperature, an increase or decrease in cooperativity (H/HHW), and a decrease in delta H of a endothermic peak) seemed to be related to biological activities.
为了阐明小分子在生物系统中的作用机制，应用差示扫描量热法（DSC）研究了22种广泛用于牙科材料和器械的脂溶性小分子与合成磷脂脂质体的相互作用。使用二肉豆蔻酰磷脂酰胆碱（DMPC）和二棕榈酰磷脂酰胆碱（DPPC）作为脂质。我们已在本期刊上报道了各种单体与脂质体的相互作用。在本实验中，研究了各种化合物，如氧化还原引发剂、光引发剂或光敏剂、抑制剂、根管消毒剂、水泥基材料（丁香酚和2-乙氧基苯甲酸）等。从这些分子引起的DMPC和DPPC脂质体的相变温度（T）、焓（ΔH kcal mol-1）和H/HHW值的变化可以得出结论，芳香叔胺、过氧化苯甲酰和对苯二酚单甲醚对脂质体有相对较大的影响，并且在酚类衍生物中，间甲酚和对氯苯酚对脂质体的影响比苯酚大。用作牙科粘固剂的丁香酚由于其高疏水性也对脂质体有很大影响。小分子引起的脂质体转变特性的变化（表现为T向较低温度的转变、协同性（H/HHW）的增加或减少以及吸热峰的ΔH的减少）似乎与生物活性有关。
