Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich (TUM), 81675 Munich, Bavaria, Germany.
STAR Protoc. 2022 May 11;3(2):101374. doi: 10.1016/j.xpro.2022.101374. eCollection 2022 Jun 17.
In this protocol, we describe the use of ChipCytometry to combine RNA hybridization and antibody staining for multiplexed tissue imaging of human formalin-fixed and paraffin-embedded tissue samples. The advantages of ChipCytometry are long-term storage for re-interrogation and advanced image quality by high dynamic range imaging of staining and background. A titrated pretreatment of tissue samples bypasses challenges because of the retrieval of antigens on coverslips and achieves an optimal staining quality at the minimal expense of tissue integrity. For complete details on the use and execution of this protocol, please refer to Jarosch et al. (2021).
在本方案中，我们描述了使用 ChipCytometry 结合 RNA 杂交和抗体染色对福尔马林固定石蜡包埋的人体组织样本进行多重组织成像的方法。ChipCytometry 的优点在于可以长期储存以备重新检测，并且通过对染色和背景的高动态范围成像获得先进的图像质量。对组织样本进行滴定预处理可以避免因在盖玻片上检索抗原而带来的挑战，并以最小的组织完整性代价实现最佳的染色质量。有关此方案的使用和执行的完整详细信息，请参见 Jarosch 等人（2021 年）。
