Granneman G R, Sennello L T
J Chromatogr. 1987 Jan 23;413:199-206. doi: 10.1016/0378-4347(87)80227-x.
A simple and extremely precise high-performance liquid chromatographic procedure has been developed for the determination of difloxacin and its metabolites in plasma and urine. Work-up of plasma samples entails ultrafiltration after addition of an internal standard in a displacing reagent containing sodium dodecyl sulfate. The ultrafiltrates are directly analyzed using a C18 reversed-phase analytical column, a soap-chromatographic mobile phase, and a fluorescence or ultraviolet detector. The mean intra-assay coefficient of variation for difloxacin over a concentration range of 10 ng/ml to 10 micrograms/ml was 0.5% when fluorescence detection and an internal standard were employed. Inter-assay coefficients of variation were approximately 2%. Recoveries of difloxacin and its metabolites were essentially quantitative and calibration curves were strictly rectilinear.
已开发出一种简单且极其精确的高效液相色谱法，用于测定血浆和尿液中的二氟沙星及其代谢物。血浆样品的处理方法是在含有十二烷基硫酸钠的置换试剂中加入内标后进行超滤。使用C18反相分析柱、皂色谱流动相和荧光或紫外检测器直接分析超滤物。当采用荧光检测和内标时，二氟沙星在10 ng/ml至10 μg/ml浓度范围内的平均批内变异系数为0.5%。批间变异系数约为2%。二氟沙星及其代谢物的回收率基本为定量，校准曲线严格呈直线。
