Bai Shu-Lin, Wang Yi-Pei, Jin Ke, Luo Xiao-Ling, Xu Xiao-Mei
Department of Orthodontics, Affiliated Stomatology Hospital of Southwest Medical University. Luzhou 646000, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2021 Dec;30(6):579-584.
To explore the influence of naringenin on osteogenic differentiation of bone mesenchymal stem cells(BMSCs), and the role of SDF-1α/CXCR4 signaling axis in the osteogenic differentiation by naringenin.
BMSCs of the rats were isolated,cultured and tested. CCK-8 assay was used to explore the proliferation ability of BMSCs in different concentrations of naringenin, and alkaline phosphatase(ALP) activity was detected. RT-qPCR was used to detect the mRNA expression of ALP, OCN, CXCR4 and SDF-1α in different groups. The expressions of CXCR4 and SDF-1α protein in BMSCs during osteogenic differentiation in different experimental groups were detected by ELISA. SPSS 21.0 software package was used for statistical analysis of the data.
The results of cell identification showed that the cultured cells were BMSCs. At 1 d and 3 d, all concentrations of naringenin had no significant effect on the proliferation of BMSCs; and at 5 d, 50 μg/mL of naringenin promoted proliferation of BMSCs；furthermore, at 7 d, all concentrations of naringenin promoted proliferation of BMSCs(P＜0.05). ALP activity value gradually increased in each concentration over time. From the RT-qPCR experiment, the mRNA expression of ALP, OCN, CXCR4 and SDF-1α in the naringenin group and the osteogenic induction group was significantly increased compared with the medium group(P＜0.05). ELISA assay showed that the protein expressions of CXCR4 and SDF-1α increased gradually in the four groups as time went on and the expression of two proteins was the highest in 100 μg/mL naringenin group.
Naringenin can promote the proliferation and osteogenic differentiation of BMSCs. SDF-1α/CXCR4 signaling axis is involved in the osteogenic differentiation of BMSCs by naringenin,particularly in the early stage of BMSCs osteogenic differentiation.
探讨柚皮素对骨髓间充质干细胞（BMSCs）成骨分化的影响，以及SDF-1α/CXCR4信号轴在柚皮素促进BMSCs成骨分化中的作用。
分离、培养和鉴定大鼠BMSCs。采用CCK-8法检测不同浓度柚皮素作用下BMSCs的增殖能力，并检测碱性磷酸酶（ALP）活性。运用RT-qPCR检测不同组中ALP、骨钙素（OCN）、CXCR4和SDF-1α的mRNA表达。采用ELISA法检测不同实验组BMSCs成骨分化过程中CXCR4和SDF-1α蛋白的表达。使用SPSS 21.0软件包对数据进行统计分析。
细胞鉴定结果显示培养的细胞为BMSCs。在第1天和第3天，所有浓度的柚皮素对BMSCs的增殖均无显著影响；在第5天，50μg/mL的柚皮素促进了BMSCs的增殖；此外，在第7天，所有浓度的柚皮素均促进了BMSCs的增殖（P＜0.05）。随着时间的推移，各浓度组的ALP活性值逐渐升高。RT-qPCR实验结果显示，与培养基组相比，柚皮素组和成骨诱导组中ALP、OCN、CXCR4和SDF-1α的mRNA表达显著增加（P＜0.05）。ELISA检测表明，随着时间的推移，四组中CXCR4和SDF-1α的蛋白表达逐渐增加，且在100μg/mL柚皮素组中两种蛋白的表达最高。
柚皮素可促进BMSCs的增殖和成骨分化。SDF-1α/CXCR4信号轴参与了柚皮素促进BMSCs的成骨分化过程，尤其在BMSCs成骨分化的早期阶段。
