雷奈酸锶对大鼠骨髓间充质干细胞成软骨分化的影响
[Effect of strontium ranelate on chondrogenic differentiation of rat bone mesenchymal stem cells].
作者信息
Yu Hao, Liu Yan, Yang Xiang-Wen, Zhang Fan, He Jia-Jing, Zhong Qun, Guo Xiao-Jing
机构信息
Shanghai Stomatological Hospital and School of Stomatology, Fudan University. Shanghai 200001, China. E-mail:
出版信息
Shanghai Kou Qiang Yi Xue. 2022 Feb;31(1):24-28.
PURPOSE
The aim of present study was to explore the effect of strontium ranelate (SrR) on the proliferation and chondrogenic differentiation of rat bone mesenchymal stem cells (BMSCs).
METHODS
Rat BMSCs were isolated and cultured in chondrogenic differentiation medium containing 0.25-2.0 mmol/L strontium ranelate. CCK-8 assay was used to study the influence of cell proliferation. Toluidine blue staining and alizarin blue staining were used to observe chondrogenic differentiation. Quantitative hydroxyproline (Hyp) activity assay was conducted. PCR and Western blots were used to detect the expression of related genes and proteins. Statistical analysis was performed using SPSS 22.0 software package.
RESULTS
0.25 mmol/L strontium ranelate did not inhibit the proliferation of BMSCs and promote the expression of chondroitin sulfate and proteoglycan. Hyp assay showed a higher content of collagen fibers in extracellular matrix in 0.25 mmol/L SrR treatment group. PCR and WB also showed up-regulated expression of Sox-9, Col-Ⅱ gene and protein, Aggrecan protein, and suppressed expression of MMP-9 gene.
CONCLUSIONS
0.25 mmol/L SrR could significantly promote chondrogenic differentiation of BMSCs.
目的
本研究旨在探讨雷奈酸锶（SrR）对大鼠骨髓间充质干细胞（BMSCs）增殖及软骨分化的影响。
方法
分离大鼠BMSCs，并在含0.25 - 2.0 mmol/L雷奈酸锶的软骨分化培养基中培养。采用CCK - 8法研究细胞增殖的影响。采用甲苯胺蓝染色和茜素红染色观察软骨分化情况。进行羟脯氨酸（Hyp）活性定量检测。采用PCR和蛋白质印迹法检测相关基因和蛋白的表达。使用SPSS 22.0软件包进行统计分析。
结果
0.25 mmol/L雷奈酸锶不抑制BMSCs的增殖，且促进硫酸软骨素和蛋白聚糖的表达。Hyp检测显示0.25 mmol/L SrR处理组细胞外基质中胶原纤维含量更高。PCR和蛋白质印迹法还显示Sox - 9、Col -Ⅱ基因和蛋白、聚集蛋白聚糖蛋白的表达上调，MMP - 9基因的表达受到抑制。
结论
0.25 mmol/L SrR可显著促进BMSCs的软骨分化。
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