J Am Mosq Control Assoc. 2022 Jun 1;38(2):83-91. doi: 10.2987/21-7054.
Blood-engorged Culex quinquefasciatus and Cx. nigripalpus were collected from 140 locations throughout Sarasota County, FL, from 2017 to 2020 to determine local, habitat-specific, and seasonal variations in the host usage patterns of these 2 important arbovirus vectors. Mosquitoes were collected using light traps, gravid traps, and via aspiration of resting shelters. Host was determined from 920 samples using multiple polymerase chain reaction protocols that target mitochondrial sequences specific to mammals, birds, and reptiles. The data were analyzed to test for statistical associations between host class and season or with habitat categories (urban, suburban, and rural). Culex quinquefasciatus took significantly more blood meals from birds compared to mammals, though a seasonal shift to a higher ratio of mammalian host usage was observed in fall. There was a habitat-dependent pattern of host usage by Cx. nigripalpus, with significantly more mammalian hosts identified from mosquitoes captured in rural habitats and a similar ratio of mammalian and avian hosts in urban habitats. In general humans were used as hosts by Cx. nigripalpus less often compared to Cx. quinquefasciatus. In contrast to previous studies, Cx. nigripalpus utilized ectothermic hosts (mostly reptiles) at a much higher ratio and exhibited no apparent seasonal shift in host selection.
从 2017 年到 2020 年，在佛罗里达州萨拉索塔县的 140 个地点收集了充血的库蚊和 Cx. nigripalpus，以确定这两种重要的虫媒病毒载体在当地、特定栖息地和季节性的宿主使用模式的变化。使用灯光陷阱、诱捕器和抽吸休息庇护所收集蚊子。使用针对哺乳动物、鸟类和爬行动物线粒体序列特异性的多重聚合酶链反应方案从 920 个样本中确定了宿主。对数据进行了分析，以检验宿主类别与季节之间或与栖息地类别（城市、郊区和农村）之间是否存在统计学关联。库蚊与鸟类相比，从哺乳动物身上摄取的血液餐明显更多，尽管在秋季观察到哺乳动物宿主使用比例的季节性上升。Cx. nigripalpus 的宿主使用存在依赖栖息地的模式，从农村栖息地捕获的蚊子中鉴定出更多的哺乳动物宿主，而在城市栖息地中，哺乳动物和鸟类宿主的比例相似。一般来说，与库蚊相比，Cx. nigripalpus 较少将人类作为宿主。与之前的研究不同，Cx. nigripalpus 利用外温宿主（主要是爬行动物）的比例要高得多，而且在宿主选择方面没有明显的季节性变化。
