Copeland J, Harman R D, Wells H G, Puckett C L
J Hand Surg Am. 1987 Mar;12(2):250-5. doi: 10.1016/s0363-5023(87)80281-2.
There is a clear need for effective methods of monitoring for postoperative occlusion of vessels in microvascular surgery. We have evaluated one technique, quantitative fluorometry, in the laboratory and clinically. Our laboratory study used rat abdominal flaps under conditions of controlled occlusion. We found accurate detection of vascular occlusion within 20 minutes, but we were unable to differentiate arterial from venous occlusion. Our clinical review of 34 microvascular cases (14 free flaps and 20 replantations) that employed fluorometry revealed corroboration of occlusion (indicated by another monitoring technique) in six cases and a diagnosis at variance with other monitoring methods in one case, thus preventing an operative exploration. We recommend the use of quantitative fluorometry as a primary or adjunctive method of monitoring when patency is in question and have outlined a protocol for clinical use.
显然需要有效的方法来监测微血管手术后血管的闭塞情况。我们已在实验室和临床中评估了一种技术，即定量荧光测定法。我们的实验室研究在可控闭塞条件下使用大鼠腹部皮瓣。我们发现在20分钟内能够准确检测到血管闭塞，但无法区分动脉闭塞和静脉闭塞。我们对34例采用荧光测定法的微血管病例（14例游离皮瓣和20例再植手术）进行的临床回顾显示，有6例经其他监测技术证实存在闭塞情况，有1例诊断结果与其他监测方法不同，从而避免了手术探查。我们建议在血管通畅性存疑时，将定量荧光测定法作为主要或辅助监测方法，并已概述了临床使用方案。
